The objective of this study was to determine the genetic diversity and virulence factors of Vibrio cholerae and V. parahaemolyticus from selected aquatic environments in Bachok, Kelantan, Malaysia. Out of 50 water samples, 33 were confirmed with the presence of presumptive V. cholerae and V. parahaemolyticus based on biochemical tests. Primers targeting toxR gene specific for V. cholerae (800bp...

wheat is a crop with spring and winter types and wide adaptability to different climate conditions. the wide adaptability of wheat is mainly controlled by three groups of genetic factors and among them vernalization (vrn) genes play pivotal role in determining spring and winter types. in this study, 395 iranian wheat landraces were characterized with specific primer pairs designed based on vrn-...

Background Diabetes Mellitus is a metabolic disorder characterized by insulin resistance with several abnormalities which trigger neuronal complications, neurodegeneration, and cognitive impairments. Repaglinide (REP) an insulinotropic, neuroprotective agent short-action poor absorption characteristics. Hence, the present study was aimed to investigate efficacy of REP-loaded lipid polymer-hybri...

Differences between endophytic and ectophytic bacterial communities with stress on antagonistic bacteria, were studied by comparing the composition of communities isolated from the rhizosphere, phyllosphere, endorhiza and endosphere of field-grown potato plants using a multiphasic approach. Terminal restriction fragment length polymorphism analysis of 16S rDNA of the bacterial communities revea...

To assess the potential for mating in several Fusarium species with no known sexual stage, we developed degenerate and semidegenerate oligonucleotide primers to identify conserved mating type (MAT) sequences in these fungi. The putative alpha and high-mobility-group (HMG) box sequences from Fusarium avenaceum, F. culmorum, F. poae, and F. semitectum were compared to similar sequences that were ...

Arbitrary amplification of polymorphic DNA sequences has increasingly been reported as a method for the genetic characterization of microorganisms, and there are many variations of this technique. Arbitrarily primed PCR (AP-PCR), random amplified polymorphic DNA (RAPD) analysis, and DNA amplification fingerprinting are the main contenders (8, 9, 79, 81). These methods all use quite different ap...