Background. Soccer, combat sports and motorcycling have all been classified as mixed in terms of their metabolism predominance thus showing a common multifaceted nature of physical requirements, which include aerobic capacity, strength, endurance, power and agility. Objectives. The aim of the current study was to compare allelic and genotype frequencies of four well-known polymorphisms among a...

Molecular markers and mapping are used to analyze an organism’s genes. They allow the selection of target genetic areas based on marker genotype (and not trait phenotype), facilitate study variability diversity, create linkage maps, follow individuals or lines carrying certain may be select parental genotypes, remove drag in back-crossing, choose difficult-to-measure characteristics. Due a lack...

Diabetic dyslipidemia is one of the leading causes of coronary artery disease (CAD) death. Genetic and environmental factors play an important role in the development of type 2 diabetes mellitus (T2DM) and dyslipidemia. The present study was aimed to investigate the association of ACE (rs4646994), FABP2 (rs1799883), MTHFR (rs1801133) and FTO (rs9939609) genetic polymorphism in T2DM with dyslipi...

A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method wa...

The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

Abstract. Ermawati D, Panjono, Bintara S, Hartatik T. 2022. Diversity of partial sequence leptin gene (Exon 3) in crossbred cattle compared to GenBank database. Biodiversitas 23: 5614-5620. Genetic marker method that is often used select Single Polymorphism Nucleotide (SNP). Polymorphisms (SNP) has been found various candidate genes, one which the gene. Leptin a affects reproduction and weight....

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. polymorphism family (GH, GHRH, and PIT1) were studied in local swamp buffalo (106 hds.) from a government breeding station (46 heads) smallholders (60 North Tapanuli District, Sumatra Province. Genotype variants three identified by PCR-RFLP method using restriction enzym...

Abstract Plasmodium vivax is the most common human malaria parasite in Asian countries including Pakistan. Present study was designed to explore genetic diversity of plasmodium genotypes based on Pvmsp-3α and Pvmsp-3βgenes using allelic specific nested PCR RFLP assays markers from field isolates district Mardan, Blood samples 200 P. malarial patients were collected after taking their written in...

Type 1 diabetes is one of the most common chronic childhood illnesses. Interplay between genetic susceptibility and environmental factors is thought to provide the fundamental element for the disease. It has been shown that more than 40 genetic loci are associated with T1DM. Important one among these is the CTLA-4. This work aimed to detect Cytotoxic T Lymphocyte-associated antigen 4 (CTLA-4) g...