In this study, the remediation performance of electrokinetic (EK) technology integrated with different surfactants for removing phenanthrene from unsaturated soils was investigated. A synthetic surfactant (Triton X-100) and a biosurfactant (rhamnolipid) were used to enhance phenanthrene solubility and removal efficiency during EK process. Results indicate that the electro-osmotic flow (EOF) rat...

Contaminant sorption within the soil matrix frequently limits biodegradation. However, contaminant bioavailability can be species-specific. This study investigated bioavailability of phenanthrene (PHE) to two PHE-degrading bacteria (Pseudomonas strain R and isolate P5-2) in the presence of rhamnolipid biosurfactant and/or a biosurfactant-producing bacterium, Pseudomonas aeruginosa ATCC 9027. Ps...

AIMS The effects and extent of adhesion of four different bacterial and two yeast strains isolated from explanted voice prostheses to silicone rubber with and without an adsorbed rhamnolipid biosurfactant layer obtained from Pseudomonasaeruginosa DS10-129 was studied. METHODS AND RESULTS The ability of rhamnolipid biosurfactant to inhibit adhesion of micro-organisms to silicone rubber was inv...

In this work, a new electrokinetic remediation technology was used to remove heavy metal and hydrocarbon from contaminated soil. The surfactant combined with PASP (Polyaspartic acid) enhance removing Electric current, soil pH, electroosmotic flow, water content variation, removal efficiency residual distribution, speciation, of TPH (total petroleum hydrocarbons), group composition oil, mechanis...

Sulfate-reducing bacteria (SRB) are widely existed in oil production system, and its H2S product inhibits rhamnolipid producing bacteria. In-situ production of rhamnolipid is promising for microbial enhanced oil recovery. Inhibition of SRB, removal of H2S and production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl were investigated. Strain Rhl can simultaneously remove S(2-) (>92%) an...

Development of environmentally benign approaches to remediation of metal-contaminated soils and sewage sludges are needed to replace currently used techniques of either landfilling or metal extraction using caustic or toxic agents. We report results from four application technologies that use a metal-chelating biosurfactant, rhamnolipid, for removal of metals or metal-associated toxicity from m...

Rhamnolipids (RL) are one of the most important classes of biosurfactants produced by microorganisms using a wide range of carbon sources, from a simple carbon source like glucose to complex wastes such as the used cooking oils used in this work. The objective of this work was to learn about the rhamnolipid-phospholipid dipalmitoyl phosphatidyl choline (DPPC) molecular interactions through the ...