To investigate analyte consumption during the laser desorption process, matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) is combined with radionuclide detection. Radionuclide detection provides highly sensitive and quantitative information on the amount of radiolabeled analytes in a MALDI MS sample spot. 14C-Labeled cytochrome c is deposited with 2,5-dihydroxybenzoic aci...

BACKGROUND Isotope dilution LC-MS/MS methods used in the clinical laboratory typically involve multi-point external calibration in each analytical series. Our aim was to test the hypothesis that determination of target analyte concentrations directly derived from the relation of the target analyte peak area to the peak area of a corresponding stable isotope labelled internal standard compound [...

The identification of binding partners of proteins by mass spectrometry following specific capture on a biosensor surface is a promising tool for proteomics research and the identification and characterization of protein-protein interactions. Previous approaches include the direct ionization of analyte from the biosensor chip on a matrix assisted laser desorption ionization time-of-flight mass ...

Background: There has been little interest in the research literature on public opinions regarding assisted conception and surrogacy, particularly in Iran. Nevertheles, the growing evidence shows that problems in adaptation and coping may be related to perceived normative values. Materials and Methods: In this cross sectional study, questionnaires on attitudes to surrogacy were completed by 187...

In this paper an imaging system for road traffic conflict analysis is proposed. The system exploits geo-referenced stereo sequences and tracking procedure to compute traffic conflict measures which can be analysed by experts. Using the potentiality of the traffic conflict technique as a surrogate safety measure could constitute an effective tool in understanding how the driver interacts and ada...

The two principal determining steps in molecular diagnosis are the amplification and the identification steps. Accuracy of DNA amplification is primarily determined by the annealing sequence of the PCR primer to the analyte DNA. Accuracy for identification is determined either by the annealing region of a labelled probe for the real time PCR analysis, or the annealing of a sequencing primer for...

A learning-based exploration approach is proposed to escape from the basins of attraction of converged-to optima, by selecting on what is termed the interestingness of a solution. This interestingness is based on the modeling error made by a surrogate model that is trained on all solutions encountered earlier during the search. Compared to multiple standard optimization runs, a learning-guided ...

Microarray-based sandwich immunoassays can simultaneously detect dozens of proteins. However, their use in quantifying large numbers of proteins is hampered by cross-reactivity and incompatibilities caused by the immunoassays themselves. Sequential multiplex analyte capturing addresses these problems by repeatedly probing the same sample with different sets of antibody-coated, magnetic suspensi...