Contents 1. Introduction 2. Transformation of dicotyledonous plants 2.1 Transformation using Agrobacterium tumefaciens 2.1.1 The role of A. tumefaciens chromosomally encoded proteins and the Vir proteins 2.2 Other transformation methods 3. Transformation of monocotyledonous plants 3.1 Biolistic transformation 3.1.1 Gene silencing 3.2 Agrobacterium transformation 4. Transformation of algae 5. Pr...

Background: Sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. One of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. Objectives: The objective of this research was to study the response of callus induc...

The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A nu...

High transformation efficiency is a prerequisite for study of gene function and molecular breeding. Agrobacterium tumefaciens-mediated transformation is a preferred method in many plants. However, the transformation efficiency in soybean is still low. The objective of this study is to optimize Agrobacterium-mediated transformation in soybean by improving the infection efficiency of Agrobacteriu...

An efficient selection system and Agrobacterium mediated transformation for chickpea (Cicer arietinum L.) cotyledonary nodes were investigated. Effect of selective agents and antibiotics on multiple shoot and root induction of cotyledonary nodes and effects of mechanical injury and vacuum infiltration on transformation efficiency were evaluated. Selective agents and antibiotics were applied to ...

The different cleavage patterns of pYBamy59 plasmid isolated from E. coli DH5alpha and B. longum MG1 by the cell extract of B. longum MG1 suggested that the main reason for low transformation efficiency was related to the restriction modification (R-M) system. To confirm correlation between R-M system and transformation efficiency, in vitro methylation and site directed mutagenesis was performe...

Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions ob...