A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic aberrations and their consequences on the transcriptome and proteome. We present an approach to extract and completely solubilize up to 98% of th...

A simple, quick and efficient method for isolating total RNA from heavy browning cells was developed by adding polyvinylpyrrolidone, mercaptoethanol and 3 M NaAc during the process of the Trizol (a kind of a widely used RNA extraction buffer) method. High-quality total RNA was isolated and synthesized to cDNA. Transcript levels of four paclitaxel biosynthetic pathway genes: dxr, hmgr, ggpps and...

Abstract Patatin-like phospholipase domain-containing protein 3 (PNPLA3) is a key lipase involved in fatty liver syndrome onset and recovery humans bovine. Regulation of PNPLA3 mediated by carbohydrate response element binding (ChREBP) rodents sterol regulatory element-binding 1c (SREBP1c) humans. The objectives our study were to optimize an siRNA knockdown ChREBP SREBP1c determine if either tr...

We read the article by Cook et al. (3) with interest, since they compared their findings with our previous work (4–8; D. Brashear, S. Taylor, J. Xiang, D. Klinzman, F. LaBrecque, M. Pfaller, B. Alden, D. LaBrecque, J. Stapleton, and W. Schmidt, 10th Triennial Int. Symp. Viral Hepatitis Liver Dis., abstr. C008, 2000; J. Xiang, F. LaBrecque, W. N. Schmidt, D. Klinzman, D. Brashear, D. R. LaBrecqu...

We read the article by Cook et al. (3) with interest, since they compared their findings with our previous work (4–8; D. Brashear, S. Taylor, J. Xiang, D. Klinzman, F. LaBrecque, M. Pfaller, B. Alden, D. LaBrecque, J. Stapleton, and W. Schmidt, 10th Triennial Int. Symp. Viral Hepatitis Liver Dis., abstr. C008, 2000; J. Xiang, F. LaBrecque, W. N. Schmidt, D. Klinzman, D. Brashear, D. R. LaBrecqu...

The emergence and distribution of drug resistance in malaria are serious public health concerns in tropical and subtropical regions of the world. However, the molecular mechanism of drug resistance remains unclear. In the present study, we performed a high-throughput RNA-Seq to identify and characterize the differentially expressed genes between the chloroquine (CQ) sensitive (3D7) and resistan...

In this study, we aimed to optimize the isolation procedure of circulating RNA from large volumes of plasma. Simultaneously, the stability and integrity of RNA from plasma samples were examined. To investigate the isolation of circulating RNA, reverse transcription-PCR analysis in combination with capillary electrophoresis was used. The presence of amplifiable RNA in plasma from healthy volunte...

Preventing protein aggregation is a major goal of biotechnology. Since protein aggregates are mainly comprised of unfolded proteins, protecting against denaturation is likely to assist solubility in an aqueous medium. Contrary to this concept, we found denatured total cellular protein mixture from mammalian cell kept high solubility in pure water when the mixture was nucleic acids free. The lys...