OBJECTIVE To establish a routine for the extraction of the total levels of CoQ10 in human plasma through the Ultra High Performance Liquid Chromatography (UHPLC). METHODS Two extraction protocols were tested: a) methanol: hexane and b) 1-propanol. The following parameters were analyzed: extraction temperature (19ºC and 4ºC), extraction tubes (glass and polypropylene), and surfactants (SDS, Tr...

In this paper, we describe a new combination method based on polytetrafluorethylene (PTFE) film-based liquid three-phase micro extraction coupled with differential pulse voltammetry (DPV) for the micro extraction and quantification of atorvastatin calcium (ATC) at the ultra-trace level. Different factors affecting the liquid-three phases micro extraction of atorvastatin calcium, including organ...

The aim of this work was to establish the best experimental conditions for the isolation of flunitrazepam and its main metabolite 7-aminoflunitrazepam from human plasma. Several mixtures of solvents (ethyl acetate, tert-butylmethyl ether, hexane, chloroform/ ethyl ether (20/80), dichloromethane / ethyl ether (20/80) and extraction cartridges (Oasis MCX , SepPak C18, Chromafix C18, Oasis HLB) we...

Multiple pesticides in water samples were successfully analyzed using an Agilent 5975T LTM GC/MSD equipped with an Agilent J&W DB-5ms Ultra Inert low thermal mass column module, with stir bar sorptive extraction and an Agilent Thermal Separation Probe for sample preparation. The method was simple, rapid, effective, and showed good linearity (R2 >0.9914) and high sensitivity for most of the targ...

Mercury (Hg) causes hazardous cumulative effects in humans; like central nervous system disorders,hypertension and chromosomal aberrations. Therefore, due to high toxicity and bioaccumulationfactor, mercury determination and speciation in human blood is very important. A sensitive,accurate, precise and inexpensive method was demonstrated for preconcentration and speciationof ultra-trace mercury...

Wastes generated from the processing of some fruits still contain large amounts of chemical components such as polyphenols, which are deemed as very effective antioxidants.This study involves the characterization of wastes generation by pink guava processing and selection of the best solvent that will allow us to extract the excess polyphenols via ultra-filtration membran...