The effects of added Si and Fe on the rate of silicic acid uptake were examined during two cruises to the equatorial Pacific upwelling zone between 110uW and 140uW. Maximum uptake rates of Si (Vmax) were highly consistent with a mean of 0.026 6 0.007 h21(n 5 29), implying maximum diatom growth rates of ,0.6 d21. Halfsaturation constants for Si uptake (KS) also showed little variance, averaging ...

Crude wild pear lipoxygenase (LOX) from ripe Kurdistan wild pears was used in this study. Extracted crude LOX was assayed spectrophotometrically for raw, ripe wild pear lipoxygenase and after 7-day storage. The effects of different buffers and pHs, substrate preparations, temperature, inhibitors and metal ions on LOX activity were evaluated at three conditions. The enzyme was most active with H...

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with res...

ABSTRACT Strong gravitational lensing observations can test structure formation models by constraining the masses and concentrations of subhaloes in massive galaxy clusters. Recent work has concluded that cluster are more abundant and/or concentrated than predicted ? cold dark matter (?CDM) simulations; this finding been interpreted as arising from unidentified issues with simulations or an inc...

Enzymes displayed on the Bacillus subtilis spore coat have several features that are useful for biocatalysis. The enzyme is preimmobilized an inert surface of coat, which due to natural sporulation process. As a result, protein stability can be increased, and they resistant environmental changes. Next, would not lyse under extreme conditions, such as in organic solvents. Furthermore, easily rem...

According with the purpose of this study, 15 professional male soccer players were recruited, and sprint Vmax 30 m measured in partials 60%, 70%, 80%, 90%, 100% intensity, using Global Positioning Systems devices (GPS). The protocol measurements was 2 days testing separated by a week. order randomized both subjects execution intensities. statistical analysis performed Student's T-test for paire...

We examined the effects of alloxan-diabetes on the kinetic properties of the soluble and the membrane-bound forms of acetylcholinesterase (AChE) in rat brain. The Km (0.15 mM) and Vmax. (1.5 mmol/min per mg of protein) of the soluble form of the enzyme were unchanged in the diabetic animals. The membrane-bound enzyme in the control group displayed a lower Km (0.09 mM) and a higher Vmax. (7.2 mm...

Results. The mean of the maximum b-wave amplitude (Vmax) at 4 weeks was 234 ± 1 4 /*V in mi "/mi" mice, which did not differ significantly from controls (266 ± 26 /iV). With increasing age, all components of the ERG decreased and by 12 weeks, the mean of the Vmax had decreased to 170 jt*V. At 18 weeks, the mean Vmax was 75 /xV, and the bto a-wave ratio was still > 1.0. Comparison of these physi...