Acetylation and other modifications on histones comprise histone codes that govern transcriptional regulatory processes in chromatin. Yet little is known how different histone codes are translated and put into action. Using fluorescence resonance energy transfer, we show that bromodomain-containing proteins recognize different patterns of acetylated histones in intact nuclei of living cells. Th...

We report a methodology that combines affinity acetylation with MS analysis for accurate mapping of an inhibitor-binding site to a target protein. For this purpose, we used a known HIV-1 integrase inhibitor containing aryl di-O-acetyl groups (Acetylated-Inhibitor). In addition, we designed a control compound (Acetylated-Control) that also contained an aryl di-O-acetyl group but did not inhibit ...

Acetylation signaling pathways are involved in numerous cellular processes and are used as therapeutic targets in several disease contexts. However, acetylated proteins only represent a minor fraction of the full proteome, and the identification and quantification of acetylated sites remain a technological challenge. Currently, pan-acetyl antibodies are used to increase the abundance of acetyla...

Abstract Background Hypertension is a prevalent chronic disease worldwide that remains poorly controlled. Recent studies support the concept gut microbiota involved in development of hypertension and dietary fibre intake may act through to lower blood pressure (BP). Resistant starch type prebiotic which metabolised by commensal bacteria colon produce short-chain fatty acids (SCFAs), including a...

Acetylation is a well-established process to improve dimensional properties of wood and resistance fungi attack. This study was carried out due limited studies on the effect acetylation mechanical properties, thermal stability, microstructural aspects wood. Wood blocks, (each 20×20×60 mm) Obeche were acetylated with acetic anhydride at 120 °C for 60, 120, 180, 240 300 minutes. The microstructur...

A rice starch based film, reinforced with starch nanocrystals (prepared by submitting native granules of rice starch to acid hydrolysis at 40 oC) was prepared by casting film-solution on leveled trays. The influence of the content of starch nanocrystals (5 to 30%) on the mechanical, thermal and structural properties of rice starch films was investigated. The rice starch films showed an increase...

The acetylation isoforms of histone H4 from butyrate-treated HeLa cells were separated by C(4) reverse-phase high pressure liquid chromatography and by polyacrylamide gel electrophoresis. Histone H4 bands were excised and digested in-gel with the endoprotease trypsin. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to characterize the level of acetylation, ...

Recognition of acetylated chromatin by the bromodomains and extra-terminal domain (BET) family proteins is a hallmark for transcriptional activation and anchoring viral genomes to mitotic chromosomes of the host. One of the BET family proteins BRD2 interacts with acetylated chromatin during mitosis and leads to transcriptional activation in culture cells. Here, we report the crystal structures ...

The eyespot of the unicellular green alga Chlamydomonas reinhardtii is a photoreceptive organelle required for phototaxis. Relative to the anterior flagella, the eyespot is asymmetrically positioned adjacent to the daughter four-membered rootlet (D4), a unique bundle of acetylated microtubules extending from the daughter basal body toward the posterior of the cell. Here, we detail the relations...