BACKGROUND Proximal cytoplasmic droplets (PCDs), a remnant of germ cell cytoplasm, are common non-specific morphological defects in bovine semen. This study evaluated the effect of higher percentages of PCDs on the quality of frozen-thawed bovine semen, embryo production and early embryo development. METHODS Three ejaculates from each of five (group 1: PCD ≤ 1%, control) and eight adult Bos i...

Boar sperm viability post-thaw differs depending on the ejaculate fraction used, with spermatozoa present in the first 10 mL of the sperm-rich fraction (SRF) (portion 1, P1, sperm-peak portion) displaying the best cryosurvival in vitro compared with that of spermatozoa from the rest of the ejaculate (portion 2 of the SRF plus the post-spermatic fraction), even when using simplified freezing rou...

BACKGROUND The glycoconjugate content of sperms indicates their physiological and fertility properties. Lectin reactivity is indicative of intact, capacitated, and acrosome-reacted sperms. In the epididymis, sperms experience maturation, glycoconjugate modification, and simultaneously, higher L-carnitine (LC) concentrations. The aim of this project was to evaluate the effects of LC and Pentoxif...

Microbiota is a cluster of physiological and pathogenic microorganisms found in many tissues organs living things, including such as bacteria, viruses, fungi. has important roles on system functions immune system, urinary digestive system. However, these can cause obstruction the genital tract, epididymitis orchitis, thus may lead to infertility. In addition, apathogenic viruses fungi have nega...

Background: This study investigated the probable effects of daily administration of methamphetamine (MA) on sperm motility, acrosome reaction (AR), acridine orange (AO), sperm chromatin dispersion (SCD) test and chromomycin A3 (CMA3) in mice. Materials and Methods: Twenty four NMRI male mice were administrated three different doses of MA intraperitoneally (n=6 in each group). Different doses of...

The divalent metal cation ionophore A23187 rapidly induces a normal acrosome reaction in a population of guinea-pig sperm suspended in calcium medium. In the course of the acrosome reaction, proacrosin, the zymogen precursor of the protease acrosin, is activated. Although the acrosome reaction causes exocytosis of the acrosomal contents, 'soluble' acrosin is not released in significant amounts ...

experiment was conducted to evaluate the effect of l-glutamine on coated sperm freezing by using four rams. semen was collected by artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. samples were pooled and centrifuged. after removing supernatant and diluting pellet, samples were split into 5 equal part and added 0, 40, 80, 120 and 160 mm l-glutamine. samples were froze...