oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. cadmium (cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. because of its long biological half-life (10–30 years), cdaccumulates in the biological systems. the present study was designed to assess the concen...

background: exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. silymarin with an antioxidant property scavenges free radicals. objective: the aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. materials and methods: ram epidydimal sper...

to determine the effect of different levels of l-glutamine and glycerol on freezing coated sperm, semen were collected from 4 taleshi rams through artificial vagina contact with a tube containing tris-fructose-egg yolk 15%. ejaculates were pooled, centrifuged and supernatant was removed, then cell fractions were used. samples were split into 9 equal parts and 3 (g3), 5 (g5) or 7% (g7) glycerol ...

The Punjab Urial (Ovis vignei punjabiensis) is an endangered subspecie of ovidae, distributed as small scattered populations in the forest belt of the Himalayan foothills of Pakistan and in the areas enclosed by the Indus and the Jhelum rivers. The present study was conducted to evaluate the liquid storage of Punjab Urial spermatozoa in different extenders for use in future in situ conservation...

introduction: in the present investigation, we aimed to study the effect of different concentrations of ascorbate/vitamin c, on membrane integrity, viability, and acrosome reaction of human spermatozoa in presence of ferrous (fe2+) ions as lipid peroxidation (lpo) promoter. material and methods: we used 12 semen samples from healthy donors in shahrekord. the samples were then washed twice with ...

Antioxidants partially ameliorated the negative effects of reactive oxygen species (ROS) produced during cryopreservation. The objective of the present study was to investigate the effect of reduced glutathione, ascorbic acid and α-tocopherol on the quality of frozen-thawed buck spermatozoa. Ejaculates were collected by artificial vagina from 8 bucks and microscopically evaluated at 37 0 C. The...

The effect of gelatin addition to the semen extender on the viability and acrosome integrity of rabbit spermatozoa was studied. Pooled semen samples were processed in a boar semen extender with or without gelatin addition. Semen samples were stored at 5 degrees C for 72 h. Viability and acrosome integrity was evaluated by light microscope. Results showed that gelatin addition had a significant ...

The season may affect the values of fresh semen variables and therefore influence the success of cryopreservation. The aim of this study was to improve the evaluation of seasonal changes in semen quality in Spanish Black Castellana roosters maintained under natural environmental conditions. Semen was collected from 11 Black Castellana roosters (housed under natural photoperiod and temperature c...

The objective of the present study was to investigate the effects of the direct addition of pentoxifylline (PF) to the ejaculates of men with poor sperm quality before freezing on post-thaw sperm motility, viability, acrosome integrity, and agonist-induced acrosome reaction. Semen specimens from 16 infertile men with impaired sperm count and motility (oligoasthenozoospermia) were divided into t...

Ninety ejaculates from a total of 76 AI boars were extended in Beltsville Thawing Solution (BTS). Boar identity, breed, weight of the ejaculate and sperm concentration were registered. Motility and acrosome integrity were assessed after storage at 16-18 degrees C for 6, 30, 54, 78, and 102 h. Storage time had a significant influence on both motility (p < 0.01) and acrosome integrity (p < 0.001)...