Background/aims—The use of “fresh” (hypothermically stored) and frozen amniotic membrane (AM) was compared in a patient with cicatricial pemphigoid with stem cell failure. The viability of both “fresh” and frozen AM epithelial cells was assessed after storage. Methods—AM was stored at either +4°C (“fresh”) or at −80°C (frozen). A “fresh” graft was applied to the cornea following superficial ker...

PURPOSE To investigate the phenotype of fetal and adult human limbal cells cultured on human amniotic membrane and the ability of cultured adult human limbal cells to repair limbal stem cell deficiency in a rabbit model. METHODS Human adult and fetal limbal cells were isolated and cultured either on plastic plates or on human amniotic membrane. Connexin43, p63, and keratins 3 and 12 (K3 and K...

The use of antimetabolites in filtration surgery, cystic, large, leaking blebs often lead to complications. We report a case of glaucoma that received amniotic membrane transplantation for repairing the exuberant bleb. This female patient had a history of several eye surgeries, including a scleral buckle and cyclocryotherapy for one eye and the other received filtering surgery. The latter had b...

We report two cases of band keratopathy who were treated with thick amniotic membrane that contained a basement membrane structure as a graft, after ethylenediaminetetraacetic acid chelation with trephination and blunt superficial lamellar keratectomy in the anterior stroma. In each case, basement membrane was destroyed and calcium plaque invaded into anterior stroma beneath Bowman's membrane. ...