The optimization of nitrogen application for root crops such as radish is important not only for yield and product quality (sponginess and hollowness) but also for the environment. Therefore, we evaluated the effect of four levels of N application (0, 150, 300 and 450 mg l-1 N) on three radish cultivars (Saxa, Red Fuoko and White Ghiaccio) grown in pots in autumn/winter (from mid-October to mid...

objective(s): despite treatment with antibiotics and vaccination with bcg, tuberculosis (tb) is still considered as one of the most important public health problems in the world. therefore, designing and producing a more effective vaccine against tb seems urgently. in this study, immunogenicity of a fusion protein which consisting or comprising cfp-10 from mycobacterium tuberculosis and the fc-...

nitrogen (n) affects adversely the tobacco yield quantity and quality as it increases yield, chlorine and nicotine contents, but decrease potassium content. this experiment was aimed at optimization of (the balance between) n concentration in leaf, stem and root to increase both yield quantity and quality (high potassium, low chlorine and medium nicotine contents) using artificial neural networ...

Background: Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function. Objectives: We introduced a nested-SOE-PCR (N –SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR.   Materials and Methods: Genomic DNA from Bacillus thermo...

Since persister cells cause chronic infections and since Escherichia coli toxin MqsR increases persisters, we used protein engineering to increase the toxicity of MqsR to gain insights into persister cell formation. Through two amino acid replacements that increased the stability of MqsR, toxicity and persistence were increased. A whole-transcriptome study revealed that the MqsR variant increas...

Worldwide, more than one million people die on the roads each year. A third of these fatal accidents are attributed to speeding, with properties of the individual driver and the environment regarded as key contributing factors. We examine real-world speeding behavior and its interaction with illuminance, an environmental property defined as the luminous flux incident on a surface. Drawing on an...