BACKGROUND Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient material for sequencing. Measuring the levels of bacterial contamination for the purposes of molecular...

Embryos and larvae of Xenopus laevis are contaminated with a wide range of micro-organisms from the maternal cloaca. Primary monolayer cultures prepared from them were usually destroyed by endogenous contaminants. Fungal contamination was readily eliminated by the usual procedures. The high incidence of bacterial contamination was not reduced by penicillin, streptomycin, tetracycline or combina...

background and aims. although wearing a white coat is an accepted part of medical and dental practice, it is a potential source of cross-infection. the objective of this study was to determine the level and type of microbial contamination present on the white coats of dental interns, graduate students and faculty in a dental clinic. materials and methods. questionnaire and cross-sectional surve...

OBJECTIVE To assess bacteriological quality of drinking water in Punjab and to evaluate usefulness of H2S strip test in comparison with multiple tube test. METHOD Samples of water were tested using H2S strips and multiple tube test. RESULTS Maximum bacterial contamination was observed in water from domestic pumps (95.83%). Followed by tap water in rural areas of Punjab (91.30%) and tap wate...

While platelet concentrates are stored at room temperature, lactic and other acids are produced and the pH decreases as the buffering capacity of the plasma is exhausted. Platelet viability will be compromised if the pH decreases to pH 6.0 and below. Similarly, a pH decrease can be produced also by bacterial contamination if the organisms produce acid as an end product. Thus the determination o...

The performance of the VersaTREK blood culture system for the detection of a bacterial contamination of platelet concentrates was assessed using samples spiked with serial dilutions of nine bacterial species associated with platelet contamination. The system detected growth for all organisms in <20 h, and the detection sensitivity was <20 CFU/ml.

The uncontrolled release of the industrial solvent methylene chloride, also known as dichloromethane (DCM), has resulted in widespread groundwater contamination in the United States. Here we investigate the role of groundwater bacterial communities in the natural attenuation of DCM at an undisclosed manufacturing site in New Jersey. This study investigates the bacterial community structure of g...

BACKGROUND Few studies have investigated the difference in bacterial contamination between conventional reused ventilator systems and disposable closed ventilator-suction systems. The aim of this study was to investigate the bacterial contamination rates of the reused and disposable ventilator systems, and the association between system disconnection and bacterial contamination of ventilator sy...