I . INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . , 2 9 SEQUENCE AND STRUCTURAL RELATIONSHIPS . . . . . . . . . . . . , 32 A. Adrenodoxin Reductase and Putidaredoxin Reductase . . . . . . . . . . . 32 H. Adrenodoxin and Putidaredoxin . , . . . . . . . . . . . . . . . . . . . . . 36 C. P450Reductase. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 8 I...

Tetracyclines, a class of antibiotics that target bacterial translation, are commonly used in research for inducible gene expression using Tet-ON/Tet-OFF systems. However, such tetracycline-inducible systems carry a risk. Given that mitochondria have a "bacterial" ancestry, these antibiotics also target mitochondrial translation and impair mitochondrial function. Indeed, treatment with doxycycl...

We know a great deal about the genes used by the model pathogen Salmonella enterica serovar Typhimurium to cause disease, but less about global gene regulation. New tools for studying transcripts at the single nucleotide level now offer an unparalleled opportunity to understand the bacterial transcriptome, and expression of the small RNAs (sRNA) and coding genes responsible for the establishmen...

The development of plasmid-mediated gene expression control in bacteria revolutionized the field of bacteriology. Many of these expression control systems rely on the addition of small molecules, generally metabolites or non-metabolized analogs thereof, to the growth medium to induce expression of the genes of interest. The paradigmatic example of an expression control system is the lac system ...

Optimal gene expression is central for the development of both bacterial systems heterologous protein production, and microbial cell factories industrial metabolite production. Our goal to fulfill industry-level overproduction demands optimally, as measured by following key performance metrics: titer, productivity rate, yield (TRY). Here we use a multiscale model incorporating dynamics (i) popu...

background: baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. the presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. methods: the aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

background : human wharton’s jelly mesenchymal stem cells (hwjmscs) express liver-specific markers such as albumin, alpha-fetoprotein, cytokeratin-19, cytokeratin-18, and glucose-6-phosphatase. therefore, they can be considered as a good source for cell replacement therapy for liver diseases. this study aimed to evaluate the effects of various culture systems on the hepatocyte-specific gene exp...