The aim of this study is to develop a standardized LC-MS/MS method for accurate measurement of desmosine (DES) and isodesmosine (IDS) in all body fluids as biomarkers for in vivo degradation of matrix tissue elastin in man and animals. A reproducible three-step analytical procedure: (1) sample hydrolysis in 6N HCl, (2) SPE by a CF1 cartridge with addition of acetylated pyridinoline as internal ...

Detection and identification of human body fluids represent very important aspects of forensic biology, since in the case of numerous offences, a firm determination of the DNA source (e.g. semen, saliva, epithelium) rather than establishing the DNA profile alone is of great significance. The presently employed routine methods are insufficient: there are no specific tests confirming the presence...

A metabolic model for cadmium has been formulated in terms of a flow scheme for cadmium among eight body compartments. The mathematical description of the flow of cadmium between compartments consits of a number of differential equations, and the accumulation of cadmium may thus be calculated. Coeffcients for the flow of cadmium were estimated from empirical data both from animals and man. The ...

Manual hemacytometer cell counting of body fluids is labor-intensive and requires skilled testing personnel. We performed a multicenter evaluation of the Iris iQ200 automated microscopy analyzer Body Fluids Module (Iris Diagnostics, Chatsworth, CA) and compared 350 iQ200 body fluid cell counts with manual hemacytometer cell counts. Within-run imprecision, expressed as coefficient of variation (...