Members of the genus Brucella are known worldwide as pathogens of wildlife and livestock and are the most common organisms of zoonotic infection in humans. In general, brucellae exhibit a range of host specificity in animals that has led to the identification of at least seven Brucella species. The genomes of the various Brucella species are highly conserved, which makes the differentiation of ...

101 isolates of Brucella spp. including the reference (n=7) and the Iranian field strains belonging to last years 1961-2003 (n=94) were biotyped and classified into 9 biotypes. Random amplified polymorphic DNA (RAPD) analysis using a ten mer-AP4 primer was generated 72 reproduciable DNA band. The typeability was 100%. The thirteen isolates were classified into five groups, each containing 2-4 b...

Lipopolysaccharides (LPS) were extracted from rough strains of Brucella abortus and Brucella melitensis and from strains of the naturally occurring rough species Brucella ovis and Brucella canis. Brucella rough lipopolysaccharides (R-LPS) were readily distinguished from Brucella smooth lipopolysaccharides (S-LPS) and enterobacterial R-LPS, by their chemical, physical, and serological characteri...

Introduction Globally, brucellosis remains one of, if not the major bacterial zoonosis, still devastating productivity of livestock and affecting mankind, either directly through infection, or indirectly through reduction of productivity among foodproducing livestock. It is estimated by the WHO that more than 500,000 new cases of human brucellosis occur each year. Many nations have deployed era...

We read with interest the paper “Ribosomal RNA Sequence Analysis of Brucella Infection Misidentified as Ochrobactrum anthropi Infection” by Horvat and colleagues (1). The paper reported that a Brucella isolate was misidentified as Ochrobactrum anthropi. The 16S rRNA results indicated that the isolates were Brucella species, with 100% agreement to rRNA sequences of known brucellae and low homolo...

results fifty (24.3%) of the 206 samples were culture positive. sh3 production was not detected in any of the isolates, and none of the isolated strains required co2. the results of the sensitivity test to basic fuchsin and thionin staining and specific agglutination and phage lysis (phage typing) tests indicated that all the isolated strains were biotype 1 b. melitansis. conclusions the cause ...

Brucellosis is an infection that affects mammals’ cells caused by a species from the genus Brucella, gram-negative, immobile, facultative, intracellular, and microaerophilic coccobacillus. In humans, pathogen are Brucella melitensis, B. abortus, suis canis. We presenting case of 62-year-old man with confirmed brucellosis his consequent cardiovascular, cutaneous, gastrointestinal, hepatic, osteo...

Bacteriophages are viruses that infect bacteria. Although their effects on bacteria have been known for many years, the discovery of antibiotics has limited widespread use bacteriophages against bacterial infections. However, it is now essential to reconsider using phage therapies due antimicrobial-resistant and uncontrolled zoonotic diseases becoming a global problem. This study aimed isolate ...

Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but not in B. canis. We report the design of a multiplex PCR test for the de...

Brucella is a Gram-negative, facultative intracellular bacterium that causes zoonotic brucellosis in humans and various animals. Out of 10 classified Brucella species, B. melitensis, B. abortus, B. suis, and B. canis are pathogenic to humans. In the past decade, the mechanisms of Brucella pathogenesis and host immunity have been extensively investigated using the cutting edge systems biology an...