نتایج جستجو برای: brucella species and biovars

تعداد نتایج: 16903694  

Journal: :Journal of clinical microbiology 1994
B J Bricker S M Halling

Several PCR assays which identify the genus Brucella but do not discriminate among species have been reported. We describe a PCR assay that comprises five oligonucleotide primers which can identify selected biovars of four species of Brucella. Individual biovars within a species are not differentiated. The assay can identify three biovars (1, 2, and 4) of B. abortus, all three biovars of B. mel...

Journal: :Veterinary microbiology 2013
Moussa Sanogo Emmanuel Abatih Eric Thys David Fretin Dirk Berkvens Claude Saegerman

Bovine brucellosis is an endemic infectious disease which can impact cattle productivity and welfare negatively, as well as human health. Sufficient knowledge on its epidemiology, particularly on species and biotypes of Brucella at national and/or regional scale are important to set up and implement efficient control measures against brucellosis in a "One health" perspective. The main objective...

Journal: :Infection and immunity 1990
B J Bricker L B Tabatabai B A Judge B L Deyoe J E Mayfield

Recently, the complete amino acid sequence of a protein expressed in Escherichia coli from cloned Brucella abortus DNA was reported. On the basis of amino acid homology, this protein was identified as a copper-zinc superoxide dismutase (Cu-Zn SOD) (B. L. Beck, L. B. Tabatabai, and J. E. Mayfield, Biochemistry 29:372-376, 1990). We demonstrate in this paper that the sequenced protein is the same...

2017
Rene Kaden Sevinc Ferrari Erik Alm Tara Wahab

BACKGROUND Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for...

2013
Yanfen Chen Yuehua Ke Yufei Wang Xitong Yuan Xiaoyan Zhou Hai Jiang Zhoujia Wang Qing Zhen Yaqin Yu Liuyu Huang Buyun Cui Zeliang Chen

BACKGROUND Human brucellosis incidence in China was divided into 3 stages, high incidence (1950-1960s), decline (1970-1980s) and re-emergence (1990-2000s). Human brucellosis has been reported in all the 32 provinces, of which Inner Mongolia has the highest prevalence, accounting for over 40% of the cases in China. To investigate the etiology alteration of human brucellosis in Inner Mongolia, th...

Brucellosis is a costly contagious disease of human, domestic and wild animals. It is a serious health problem in Iran causing significant economic losses therefore, control approaches to prevent its spread are of great importance. In Iran, the species and biovars of virulent Brucella species are still under-reported due to the inadequate diagnostic protocols and insufficient laborator...

Journal: :Journal of clinical microbiology 1991
M J Corbel

Strains of Brucella melitensis biovars 1, 2, and 3 isolated in India, Italy, Kuwait, Saudi Arabia, the Federal Republic of Germany, and Zimbabwe were inhibited by thionin (20 micrograms/ml) but not by basic fuchsin (20 micrograms/ml) or safranin O (200 micrograms/ml). In other respects the strains were typical of their species and biovars. The existence of dye-sensitive strains of B. melitensis...

Journal: :jundishapur journal of microbiology 0
reza mirnejad molecular biology research center, baqiyatallah university of medical sciences, tehran, ir iran mozafar mohammadi molecular biology research center, baqiyatallah university of medical sciences, tehran, ir iran ali majdi young researchers club, tonekabon branch, islamic azad university, tonekabon, ir iran niloufar taghizoghi bounty house, stowage, bellerbys college, london, united kingdom vahhab piranfar department of biology, tonekabon branch, islamic azad university of tonekabon, tonekabon, ir iran; department of biology, tonekabon branch, islamic azad university of tonekabon, tonekabon, ir iran. tel: +98-937 130 7300, fax: +98-2188039883

conclusions the results of this study showed that pcr–rflp technique was a fast and applicable method especially for separation, detection and differentiation between species of b. melitensis and b. abortus biovars in blood sample. also the presented data showed that b. melitensis biovar 1 was the prevalence biovar. background brucella is an intracellular parasite of the disease brucellosis thr...

Journal: :Journal of clinical microbiology 2000
B J Bricker D R Ewalt A P MacMillan G Foster S Brew

Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 isolates from marine mammals showed all...

Journal: :Journal of clinical microbiology 2000
L Fernández-Lago F J Vallejo I Trujillano N Vizcaíno

A whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16S rRNA sequence of Brucella abortus in combination with flow cytometry has been developed. With the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of Brucella and with a total of nine different Brucella clinical isolates. U...

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