l-asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.in this study, simple and advantageous method has been validated for rapid and precise determination of intracellular l-asparaginasein bacterial species. a suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

in this study, silver nanoparticles were synthesized  using the fungus, aspergillus fumigatus. the effects of three independent variables including glucose content of culture media, initial ph and initial spore concentration on biosynthesis of silver nanoparticles were investigated. these variables affect cell morphology, cell mass, size and morphology of silver nanoparticles and degree of silv...

abstract   introduction: neurodegeneration change is one of the hallmark symptoms of which alzheimer’s disease (ad) can be modeled by β-amyloid injection into specific regions of brain. (-)-epigallocatechin-3-gallate (egcg) is a potent antioxidant agent that its role against oxidative stress and inflammation has been shown in prior studies. in the present study, we have wanted to determine whet...

for all industrial processes, modelling, optimisation and control are the keys to enhance productivity and ensure product quality. in the current study, the optimization of process parameters for improving the conversion of isoeugenol to vanillin by psychrobacter sp. csw4 was investigated by means of taguchi approach and box-behnken statistical design under resting cell conditions. taguchi desi...

Objective(s): In previous studies, antioxidant activity of Viola odorata L. has been demonstrated. In this study, we have investigated the anti-melanogenic effect of extract and fractions of the plant in B16F10 cell line. Materials and Methods: Impact of different increasing concentrations of extract and fractions of V. odorata was evaluated on cell viability, cellular tyrosinase, melanin conte...

A fermentation process for production of super coiled plasmid DNA in E. coli for use as a DNA vaccine was developed. In the process a feed-back control nutrient feeding strategy based on pH and DO was used to regulate the cell growth rate by controlling the interactivity of the nutrient feed rate, pH and DO. The process increased the total yield of plasmid DNA by approximately 4 fold as compare...

introduction: decidua as the nearest mother's tissue layer to the fetus which is in direct contact with the embryo has an important role in composing the appropriate microenvironment for protection of allogenic fetus from mother's immune response. in this study the effect of supernatant from culture of decidual cells on dendritic cells regarding the induction of t-cell allogenic proliferation r...