The culture of two Kluyveromyces species, Kluyveromyces lactis (ATCC 96897) and Kluyveromyces bulgaricus (ATCC 96631), in the presence of subinhibitory doses of amphotericin B leads to the selection of mutants which are resistant to this polyene. The mutants show an alteration of their cell wall composition with the main change corresponding to an increase of chitin. The enzyme activities invol...

abstract aims: the increasing usage of antibiotics can cause resistance to the treatment of infections, which can caused by bacteria, e.g. pseudomonas aeruginosa. the aim of this study was to trace the class i, ii and iii integrons in isolates of p. aeruginosa of nosocomial infection and determining the antibiotic resistance pattern of the bacteria. instrument & methods: in this cross-sectional...

Table of Contents 1 Introduction 1 1.1 Structure and function of chitinases 1 1.2 Filarial chitinases are potential targets for interventions 4 1.3 A. viteae as a model to investigate parasitological parameters of filarial diseases 6 1.4 Aims and objectives of the study 7 2 Results 9 2.1 Characterisation of A. viteae chitinase genes 9 2.1.1 Determination of the gene structure of A. viteae chiti...

The chitinase producing ability of Pseudomonas fluorescens strains viz., PF1, PB2 and FP7 was evaluated in a culture medium with and without a chitin source. The addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP7 responded well to the addition of chitin by producing 31.2% increased chitinase in c...

A citrus cDNA encoding a class II acidic chitinase was isolated from a nonembryogenic cell line of sweet orange using the tobacco cDNA clone PROB3. Northern blot analysis revealed that the corresponding mRNA is expressed in young, green bark but not in leaves, roots, or flavedo.

Background and Aims: The indiscriminate use of antibiotics can lead to antibiotic resistance in the treatment of infections caused by bacteria such as Pseudomonas aeruginosa. The presence of integrons in Pseudomonas is clearly associated with multidrug resistances. Therefore, this study aimed at tracking class I, II and III integrons of antibiotic-resistant isolates of Pseudomonas aeruginosa th...

In the present study, we identified chitinase genes in the genome of mulberry for the first time. The mulberry genome encodes 20 chitinase genes, which are grouped into two main families and organized into five classes. The genomic structures and phylogenetic relationships of mulberry chitinase genes were analyzed, which provided a genetic basis for understanding the functions of these genes. W...

Chitinase A of Streptomyces cyaneus SP-27 or chitinase I of Bacillus circulans KA-304 showed the protoplast-forming activity when combined with alpha-1,3-glucanase of B. circulans KA-304. The gene of chitinase A was cloned. It consisted of 903 nucleotides encoding 301 amino acid residues, including a putative signal peptide (35 amino acid residues). The deduced N-terminal moiety of chitinase A ...