نتایج جستجو برای: cmcase assay

تعداد نتایج: 221846  

Journal: :Bioresource technology 2009
Cheng-Hsiung Chang Shang-Shyng Yang

In order to prepare the multi-functional biofertilizer, thermo-tolerant phosphate-solubilizing microbes including bacteria, actinomycetes, and fungi were isolated from different compost plants and biofertilizers. Except Streptomycesthermophilus J57 which lacked pectinase, all isolates possessed amylase, CMCase, chitinase, pectinase, protease, lipase, and nitrogenase activities. All isolates cou...

2013
Diogo Robl Priscila da Silva Delabona Carla Montanari Mergel Juan Diego Rojas Patrícia dos Santos Costa Ida Chapaval Pimentel Vania Aparecida Vicente José Geraldo da Cruz Pradella Gabriel Padilla

BACKGROUND There is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. One of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. The foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2G). New cellulol...

Journal: :basic and clinical cancer research 0
majid mahmoodi cancer research center, tehran university of medical sciences, tehran, iran. farshid farhan radiotherapy oncology research center, cancer institute of iran, tehran university of medical sciences, tehran, iran. cyrus azimi cancer research center, cancer institute of iran, tehran university of medical sciences. tehran, iran. mohammad-ali mohagheghi cancer research center, cancer institute of iran, tehran university of medical sciences. tehran, iran. farideh farzanfar cancer research center, cancer institute of iran, tehran university of medical sciences. t azam noormohammadi radiotherapy oncology research center, cancer institute of iran, tehran university of medical sciences, tehran, iran.

purpose: the aim of this study was to test the in vitro sensitivity of lymphocytes of patients with head and neck cancer against gamma irradiation and also to find out if the frequencies of chromosomal aberrations correlate with side effects of radiotherapy. patients and methods : peripheral blood of 101 patients with head and neck cancer was collected before the onset of radiotherapy, cultured...

Journal: :Hayati Journal of Biosciences 2023

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates obtained, and thirty-three showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by C12, identified as Leclercia adecarboxylata based 16S-rRNA gene sequence analysis. SDS-PAGE of C12 ce...

2013
E. P. Macedo C. L. O. Cerqueira D. A. J. Souza A. S. R. Bispo R. R. R. Coelho R. P. Nascimento

Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentat...

2012
Ling Lin Xianzhao Kan Hao Yan Danni Wang

The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28oC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40oC and pH 4.0, resp...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه صنعتی اصفهان - دانشکده کشاورزی 1389

سلولز فراوان ترین پلیمر زیستی روی زمین است و تقریباً 50% بیوسفر را تشکیل می دهد. این هوموپلیمر خطی از واحدهای d-گلوکز تشکیل شده است که توسط پیوند های بتاگلیکوزیدی به هم متصل می شوند. سلولز توسط آنزیم های تجزیه کننده سلولز یا سلولازها، به گلوکز تبدیل می گردد. سلولاز یک سیستم آنزیمیست که از سه گروه آنزیمی اندوگلوکاناز، اگزوگلوکاناز و بتاگلوکوزیداز تشکیل شده است. اندوگلوکاناز با تجزیه تصادفی پیونده...

Journal: :مجله پزشکی مولکولی 0
abbas ebrahimi- kalan department of neurosciences, faculty of advanced medical sciences, tabriz university of medical sciences, tabriz, iran shahram ahmadian clinical laboratory, imam reza hospital, tabriz university of medical sciences, tabriz, iran hamid tayefi-nasrabadi department of anatomical, faculty of medicine, tabriz university of medical sciences, tabriz, mohammad pourhassan-moghaddam department of medical biotechnology, faculty of advanced medical sciences, tabriz university of medical sciences, tabriz, iran c ian wark research institute, mawson lakes campus, university of south australia, mawson lakes,

introduction: telomeric repeat amplification protocol assay (trap assay) is a standard established technique to detect the activity (expression) of telomerase in the cancerous samples. it includes a time-consuming procedure that has significant drawbacks such as high cost, complicacy, time-consuming procedure, equipment-dependent steps and false-positive signals caused by inclusion of pcr insid...

Journal: :Concilium 2023

The worldwide production of microbial enzymes is generally limited by the cost substrate used for cultivation microorganisms. Thinking a low-cost and abundant lignocellulosic substrate, coconut fiber an alternative because it rich in cellulose, hemicellulose, lignin, presenting itself as promising raw material enzyme cocktail, which can be second-generation biofuels. objective this work was to ...

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