Background: Activation of bone morphogenetic protein 4 (BMP4) signaling pathway in embryonic stem (ES) cells plays an important role in controlling cell proliferation, differentiation, and apoptosis. Adverse effects of BMP4 occur in a time dependent manner; however, little is known about the effect of different time exposure of this growth factor on cell number in culture media. In this study, ...

Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...

This study presents effects of some nutritional and environmental factors on cellulases production by isolates, Bacillus licheniformis WBS1 and Bacillus sp. WBS3, which have been isolated from an Indian hot spring. Activity of both CMCase and FPase were higher in case of wheat straw. Maximum enzymatic activity was found at following optimum parameters: pH (8.0 when grown on rice, 9.0 when grown...

In the present study rotifer Brachionus plicatilis was cultured under controlled laboratory conditions, in Marine Science Center, Dept. of Biology, temperature 22 ºC. The rotifers feeding on two type food, first baker's yeast and second animal manure. General, used manure better than yeast. density culture at ºC which fed 145 ind /ml, while growth rate 2.67 doubling time 0.25 day. While, densit...

introduction: cyclosporin a (cya) is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of t–cell activation. several studies have reported the synergistic effect of cya with hematopoietic growth factor in colony culture of mice bone marrow. we studied the effect of il-3, il-6, scf,cya and nutrition medium ( condition medium - pha) on the developme...