Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...

Objective: The role of growth factors, including vascular endothelial growth factor of activated omentum on mitosis is clearly known, though not on all the aspects of in vitro oocyte maturation. This study was designed to assess the effect of activated-omental extract (AOE) on in vitro maturation (IVM) of rat cumulus-oocyte complexes (COCs). Materials and Methods: In this experimental study, th...

objective(s) nonviral vector can be an attractive alternative to gene delivery in experimental study. in spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. to determine the effective expression, the recombinant escherichia coli lacz genes were cloned into the different variants of pcdna3.1 and then...

our previous studies revealed an inhibitory effect of icd-85 (venom derived peptides) on breast cancer cell line mda-mb231. icd-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. however, the exact mechanism of icd-85 was unknown. hence, the present study was undertaken to assess the mechanism of icd-85 effect as an anti-proliferative agent of cancer cells. the effec...

since most of the studies on chemical compounds of dermatophytes have shown the existence of a relationship between their pathogenisity and proteolytec enzymes. activities of 19 different enzymes in viable mycelia and cytoplasmic extracts of t.rubrum (cetr), t.mentagrophytes (cetm) and t.verrucosum (cetv) were investigated by the api-zym system. the results showed that viable mycelia of t.rubrm...