A simple and Reliable Bench top microscope is constructed to detect the fluorescence intensity for quantum dot. Which can act as fluorescent tag a sensitive studies like sandwich enzyme-linked immunosorbent assay (ELISA). The Cadmium Telluride (CdTe) capped with MPA (3-Mercaptopropionic acid) dot signal output (Excitation: 490 nm, Emission: 515 nm) used. It bifunctional molecule containing both...

A simple, sensitive and cost-effective method for the analysis of the mycotoxin aflatoxin B1 (AFB1) has been established based on controlled growth of immunogold. AFB1-BSA conjugate modified magnetic beads were employed as capture probe and anti-AFB1 antibody-coated gold colloids were used as detection probe for the immunological recognition of AFB1, as well as for signal transduction. The immu...

Toxocaracati is one of the most important and widespread of the helminth zoonosis. In the present study, helminthosis due to Toxocaracati in 55 stray cats in Tehran was studied by necropsy. In addition, two different diagnostic methods, including serological and coprological tests, for infection with this parasite were compared. The dot-ELISA assay used Toxocaracati crude antigens to evaluate t...

OBJECTIVE To evaluate whether a new fluid-phase filtration radioassay possesses both high sensitivity and specificity compared with the currently used ELISA and Farr assays. METHODS Sequential sera (25 samples) from 9 patients with systemic lupus erythematosus (SLE), sera from 20 patients with SLE possessing anti-dsDNA antibodies by the Crithidia assay, 75 patients with rheumatoid arthritis p...

Background and Objective: Today, one of the problems of health systems is the presence of cockroaches in hospitals as insects that move freely in and out of the hospitals and are infected with pathogenic bacteria. The aim of this study was to identify carbapenem resistance genes in Escherichia coli isolated from Blattella germanica by dot blot assay in Hamadan hospitals in the west of Iran. Me...

Legionella pneumophila is an intracellular pathogen that multiplies in a specialized vacuole within host cells. Biogenesis of this vacuole requires the Dot/Icm type IV protein translocation system. By using a Cre/loxP-based protein translocation assay, we found that proteins translocated by the Dot/Icm complex across the host phagosomal membrane can also be transferred from one bacterial cell t...

In the present study, generation and characterization of a highly specific monoclonal antibody (mAb) against Ochratoxin A (OTA) was undertaken. The generated mAb was further used to develop a simple, fast, and sensitive sandwich dot-ELISA (s-dot ELISA) method for detection of OTA from contaminated food grain samples. The limit of detection (LOD) of the developed enzyme-linked immunosorbent assa...

The results presented here record the first confirmation of bluetongue virus (BTV) antibody in sheep and goats in Kerala State. A total of 1,010 sera collected from the 14 districts within the state were screened for the presence of group-specific BTV antibodies by dot enzyme-linked immunosorbent assay (dot ELISA). Positive samples were obtained from 12 of the 14 districts. The overall BTV anti...

A dot enzyme-linked immunosorbent assay utilizing a novel membrane, polyvinylidene difluoride, is described. This assay was developed for the rapid detection of serum antibodies to eastern equine encephalomyelitis virus and St. Louis encephalitis virus in sentinel chickens. Antigens were spot-filtered through the membrane. Membranes were dipped into small vials of sera. Antigen-antibody complex...