OBJECTIVE The correlation of the embryo matrix metalloproteinase-9 (MMP-9) secretion, embryonic development and clinical pregnancy was investigated. MATERIALS AND METHODS The embryo culture from in vitro fertilization-embryo transfer (IVF-ET) patients were collected in the Xuzhou Central Hospital from January 2013 to September 2014. At the same time, the embryo grade was recorded. The secreti...

Morphology and cleavage rate remain the mainstay of embryo assessment. However, a number of additional technologies for this application are under investigation. These include the measurement of glucose, lactate, pyruvate or amino acid levels in the embryo culture media, assessment of oxygen consumption by the embryo, genomic and proteomic profiling, and most recently, analytical examination of...

Vitamin A (all-trans retinol) is an important antioxidant whose role in embryo development in vitro and in vivo is well established. Oxidative stress is a major cause of defective embryo development. This study evaluated the effects of all-trans retinol supplementation to maturation and embryo culture media under different gaseous environments on the development of ovine oocytes and embryos in ...