background: in this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. methods: the developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of 20 ...

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

the major objective of seedless grape breeding program is production of grapes with high quality. breeding of seedless grapes is difficult because the embryos are aborted after fertilization. embryo rescue technique is utilized to obtain plants from crosses between seedless cultivars. in the present study, the effect of paternal genotypes and different iaa concentrations in presence of ba appli...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

taxus chinensis var. mairei is a rare and endangered medicinal plant species distributed in china. in order to promote fast propagation and preserve the natural resources, conditions for in vitro germination and seedling development of embryos of t. chinensis var. mairei from anhui or zhejiang were investigated. results showed that in vitro germination rate of excised embryos cultured under 14 ...

The success rate of embryo production critically depends on several factors. One of the pivotal factors is a technique used for the whole procedure of in vitro fertilization (IVF) and in vitro embryo culture (IVC) (1, 2). Besides the technique selected for IVF / in vitro embryo production (IVEP), there is an important consideration called IVF/IVEP culture dishes disin...

common yew taxus baccata l. is an evergreen, slow growing   tree, indigenous of hyrcanian forests in iran. discovery of paclitaxel as an   anti tumor agent in the bark of taxus has increased importance of this   plant for treatment of breast and ovary cancer, especially 7000 women die annually   due to breast cancer in iran. regarding destructive method of paclitaxel   production and protection...

background: the basal medium that supports isolated mouse oviduct (imo) is important for supporting embryo development and quality. methods: the culture of ovine ivm/ivf zygotes was done in imo using sofaacibsa and sofaabsa as basal medium of imo and in sofaabsa alone as control. for preparation of imo mature inbred strain c57bl/6 female mice were synchronized and mated with vasectomized males....

abstract background: research studies on reproductive mechanism of laboratory animals are essential for further advancement of assisted reproductive techniques (art). one of these studies includes the assessment of in-vitro development of pre-implantation embryos. the objective was to compare the cleavage rates and morphology of in-vivo formed 2 to 8 cell embryos and blastocysts with in-vitro c...

most of the systematic studies used in the development of human embryo culture media have been done first on mouse embryos. the general use of nmri outbred mice is a model for toxicology, teratology and pharmacology. nmri mouse embryo exhibit the two-cell block in vitro. the objective of this study was to evaluate and compare the effects of four kinds of culture media on the development of zygo...