An original 1-acetato-4-(1-pyrenyl)-1,4,7-triazacyclononane (AcPyTACN) was synthesized for the immobilization of a His-tagged recombinant CODH from Rhodospirillum rubrum (RrCODH) on carbon-nanotube electrodes. The strong binding enzyme at Ni-AcPyTACN complex affords high current density 4.9 mA cm−2 towards electroenzymatic CO2 reduction and stability more than 6×106 TON when integrated gas-diff...

wet ag/agcl electrodes, although very popular in clinical diagnosis, are not appropriate for expanding applications of wearable biopotential recording systems which are used repetitively and for a long time. here, the development of a low‑cost and low‑noise active dry electrode is presented. the performance of the new electrodes was assessed for recording electrocardiogram (ecg) and electroence...

Abstract Research has been carried out on the manufacture of tungsten indicator electrodes with a diameter 1 mm coated PVA-Enzyme PVC-KTpClPB using biosensor potentiometer method. It sensitivity 19,096 mV/decade, detection range 1.10 -5 - 5.10 -4 M, limit M and correlation coefficient R 2 = 0.9431. This is very small, only difference one order to negative power. On basis this small range, PVA-E...

Minimizing overpotential and generating high faradaic currents are critical issues for fast-scan voltammetry of beta-nicotinamide adenine dinucleotide (NADH) for the sensitivity of enzyme-modified electrodes based on dehydrogenases. Although NADH voltammetry exhibits high overpotential and poor voltammetric peak shape at solid electrode surfaces, modification of the electrode surface can improv...

BACKGROUND The CombiMatrix ElectraSense microarray is a highly multiplex, complementary metal oxide semiconductor with 12,544 electrodes that are individually addressable. This platform is commercially available as a custom DNA microarray; and, in this configuration, it has also been used to tether antibodies (Abs) specifically on electrodes using complementary DNA sequences conjugated to the A...

• Adsorbed-enzyme-mediated electrochemical processes are investigated via simulations. Potential dependent Michaelis constants explained. Four classes of voltammograms introduced to help understand current responses. Porous electrodes can create enhanced effective surface coverage and sensing ability. We present theory for electrolysis at electrode surfaces modified by a layer electroactive enz...

The present study focuses on fragmented deglycosylated pyranose dehydrogenase (fdgPDH) from Agaricus meleagris recombinantly expressed in Pichia pastoris . Fragmented deglycosylated PDH is formed from the deglycosylated enzyme (dgPDH) when it spontaneously loses a C-terminal fragment when stored in a buffer solution at 4 °C. The remaining larger fragment has a molecular weight of ∼46 kDa and ex...

Redox cycling of enzymatically amplified electroactive species has been widely employed for high signal amplification in electrochemical biosensors. However, gold (Au) electrodes are not generally suitable for redox cycling using a reducing (or oxidizing) agent because of the high background current caused by the redox reaction of the agent at highly electrocatalytic Au electrodes. Here we repo...

We report the functionalization of multi-walled carbon nanotube (MWCNT) electrodes by oxidative electropolymerization of pyrrole monomers bearing pyrene and N-hydroxysuccinimide groups. Both polymers were applied to the immobilization and electrical wiring of Trametes versicolor laccase via chemical grafting or non-covalent binding. A "pseudo" host-guest interaction of polymerized pyrene with a...

A key challenge for sensor miniaturization is to create electrodes with smaller footprints, while maintaining or increasing sensitivity. In this work, the electroactive surface of gold was enhanced 30-fold by wrinkling followed chronoamperometric (CA) pulsing. Electron microscopy showed increased roughness in response an number CA pulses. The nanoroughened also excellent fouling resistance when...