presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme substrat...

Neste trabalho de revisão são analisados os avanços mais recentes em eletrodos enzimáticos amperométricos dando ênfase particular aos biosensores baseados na Glucose Oxidase e na Horseradish Peroxidase. A intermediação redox através de mediadores artificiais solúveis ou ligados a polímeros é discutida em termos dos desenvolvimentos teóricos recentes e verificações experimentais. É analisada a d...

Presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. Bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme subs...

A physic-mathematical model has been developed that is suitable for biosensors incorporating membranes and bisubstrate enzymes. The set of equations regarding the transport of reactants across the membrane, the bisubstrate enzymatic reaction, back diffusion of reactants and reaction products, are written down and simultaneously solved and transient response of the electrode is determined as...

a physic-mathematical model has been developed that is suitable for biosensors incorporating membranes and bisubstrate enzymes. the set of equations regarding the transport of reactants across the membrane, the bisubstrate enzymatic reaction, back diffusion of reactants and reaction products, are written down and simultaneously solved and transient response of the electrode is determined as a f...

We present a study of electrocatalysis by an enzyme adsorbed on a range of carbon materials, with different size, surface area, morphology and graphitic structure, which are either commercially available or prepared via simple, established protocols. We choose as our model enzyme the hydrogenase I from E. coli (Hyd-1), which is an active catalyst for H2 oxidation, is relatively robust and has b...

Abstract Redox polymers have been widely used to facilitate and stabilize bioelectrochemical communication between the active sites of enzymes electrodes, enabling development high-performance enzyme electrodes for sensing, provision power, in synthesis. This review offers a brief overview recent efforts use additional polymer layers on top redox polymer/enzyme layer, which can both improve per...

choline oxidase (chox) was chosen as a model enzyme for evaluating the performance of cnts’ functional groups for development of enzyme electrodes. cnts were functionalized with carboxylic acid, amine or amide groups. carboxylic acid, amine and amide functionalized cnts were obtained by acid treatment, ethylenediamine or tetraethylenepentamine chemically modification and ammonia plasma treatmen...