A rapid process for purification of an extracellular beta-xylosidase with high purity was developed. The manipulation involved the precipitation of protein from culture medium and the extraction of enzyme from the resuspended crude protein solution by an aqueous-two phase separation. A linear random copolymer, PE62, with 20% ethylene oxide and 80% propylene oxide was employed in both stages of ...

Small-angle neutron scattering and mean-field lattice modeling were used to characterize a class of water-based magnetic fluids tailored specifically to extract soluble organic compounds from water. The fluids consist of a suspension of approximately 7 nm magnetite (Fe3O4) nanoparticles coated with a bifunctional polymer layer comprised of an outer hydrophilic poly(ethylene oxide) (PEO) region ...

We demonstrate that broadband coherent anti-Stokes Raman scattering (CARS) microscopy can be very useful for fast acquisition of quantitative chemical images of multilayer polymer blends. Since a raw CARS signal results from coherent interference of resonant Raman and nonresonant background, its intensity is not linearly proportional to the concentration of molecules of interest, and it is chal...

Pluronic F127 polyol, a block copolymer of propylene oxide and ethylene oxide, was studied as an alternative to agar in culture media for nematodes, bacteria, fungi, actinomycetes, and plant tissues or seedlings, At a polyol concentration of 20% w/v, the culture media, semi-solid at room temperature (22 C) but liquid at lower temperatures, had minimal effects on the test organisms. Most of the ...

In this paper, the Maxwell–Wagner effect and charge characteristics of heterogeneous interface at action higher electric field elevated temperature are investigated by means electret technology. A composite membrane with a double-layer structure polypropylene (PP) film fluorinated ethylene propylene copolymer (FEP) was made. After being polarized under temperature, component PP FEP films membra...

We report the design, synthesis, morphology, phase behavior, and mechanical properties of semicrystalline, polyolefin-based block copolymers. By using living, stereoselective insertion polymerization catalysts, syndiotactic polypropylene-block-poly(ethylene-co-propylene)-block-syndiotactic polypropylene and isotactic polypropylene-block-regioirregular polypropylene-block-isotactic polypropylene...

In situ aerobic cometabolic transformations of ethylene, propylene, and cis-1,2-dichloroethylene (c-DCE), by microorganisms stimulated on propane, were examined in groundwater contaminated with c-DCE and trichloroethylene (TCE). In situ measurements were performed by conducting field push-pull tests, which consisted of injecting site groundwater amended with a bromide tracer and combinations of...

This study developed single-well, gas-sparging tests for assessing the feasibility of in situ aerobic cometabolism of trichloroethene (TCE) and cis-1,2-dichloroethene (cis-DCE) using propane and methane as growth substrates. Tests were performed in groundwater contaminated with TCE (100-400 microg l(-1)) and cis-DCE (20-60 microg l(-1)). A series of gas-sparging tests was performed by first spa...

Reanalysis of an asymmetric poly(ethylene-alt-propylene)-block-polydimethylsiloxane (PEP-PDMS) diblock copolymer first investigated in 1999 has revealed a rich phase behavior including dodecagonal quasicrystal (DDQC), Frank–Kasper σ phase, and body-centered cubic (BCC) packing at high temperature adjacent to the disordered state. On subjecting sample large amplitude oscillatory shear well below...