نتایج جستجو برای: explants

تعداد نتایج: 7790  

Journal: :Horticulturae 2023

Cryopreservation features of Lithuanian garlic (Allium sativum L.) cultivars ‘Žiemiai’ and ‘Dangiai’ were investigated. The survival rate state explants, depending on the type explant, dehydration conditions evaluated in experiment. Explants isolated from cloves bulbils dehydrated with a plant vitrification solution 3 (PVS 3) containing 50% w/v glycerol sucrose liquid MS medium. Three different...

Mahlagha Ghorbanli, Roksana Saleh Sedighe Khalife

Thymus daenensisis a perennial plant belonging to Lamiaceae family that is a species endemic to Iran. Due to the indiscriminate use of this plant which possesses medicinal values, T. daenensis tissue culture is suggested to increase the quality and quantity of its effective compounds. Callus cultures using leaf and stem explants were subjected to different hormonal treatments. Ten-millimeter ex...

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented withdifferent concentrations of N6-benzyladenine (BA) (0.5, 1, ...

Journal: :The American journal of sports medicine 2010
Liming Bian Aaron M Stoker Kevin M Marberry Gerard A Ateshian James L Cook Clark T Hung

BACKGROUND Intact articular cartilage tissue is used clinically in the form of osteochondral allografts and experimentally as explants in modeling the physiologic behavior of chondrocytes in their native extracellular matrix. Long-term maintenance of allograft tissue is challenging. HYPOTHESIS By carefully modulating the preservation environment, it may be possible to preserve osteochondral a...

Journal: :Circulation research 1989
D Demolle A Van Coevorden J M Boeynaems

It has been shown previously that serotonin stimulates the production of prostacyclin by bovine aortic smooth muscle cells in culture, via 5-HT2 receptors (Coughlin SR, Moskowitz MA, Antoniades HN, Levine L. Proc Natl Acad Sci USA 1981;78:7134-7138). These cells express a synthetic phenotype, whereas the majority of the smooth muscle cells in the media from adult arteries are in a contractile s...

2015
Christopher H. Lieu Peter J. Klauck Patrick K. Henthorn John J. Tentler Aik-Choon Tan Anna Spreafico Heather M. Selby Blair C. Britt Stacey M. Bagby John J. Arcaroli Wells A. Messersmith Todd M. Pitts S. Gail Eckhardt

BACKGROUND CRC is a significant cause of cancer mortality, and new therapies are needed for patients with advanced disease. TAK-733 is a highly potent and selective investigational novel MEK allosteric site inhibitor. MATERIALS AND METHODS In a preclinical study of TAK-733, a panel of CRC cell lines were exposed to varying concentrations of the agent for 72 hours followed by a sulforhodamine ...

2016
Sandhya Srikanth Tsui Wei Choong An Yan Jie He Zhong Chen

Plant propagation via in vitro culture is a very laborious and time-consuming process. The growth cycle of some of the crop species is slow even in the field and the consistent commercial production is hard to maintain. Enhanced methods of reduced cost, materials and labor significantly impact the research and commercial production of field crops. In our studies, stem-segment explants of Brassi...

2009
De Wilde R Rama Subbu

In vitro clonal propagation of Ashoka, Saraca asoca (Roxb.) De Wilde plant was achieved using shoot tip, nodal and internodal explants. The explants were cultured on MS medium supplemented with different concentrations (0.5-2.0mg/l) of Benzyl amino purine (BAP), Kinetin (Kn) and 2, 4-Dichlorophenoxy acetic acid (2,4-D). The synergetic effect of BAP (0.5mg/ l) induced a mean of 11.71±0.53 advent...

Journal: :Indian journal of experimental biology 2002
A N Pyati H N Murthy E J Hahn K Y Paek

In vitro propagation of Dendrobium macrostachyum, a threatened and endemic species was achieved through nodal explants. The nodal explants were cultured on Murashige and Skoog (MS) basal medium and MS medium supplemented with N6-benzyladenine (BA-2.22, 4.44 and 8.88 microM), Kinetin (KN-2.32, 4.65, and 9.29 microM) and Coconut water (CW, 5, 10 and 15%) individually or in combination with 2.69 m...

2016
TATJANA BAKRAN-PETRICIOLI SIBILA JELASKA

Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404 strain) was performed in two ways: in vitro by the transformation of root and stem explants and inplanta by the transformation of whole plants in the green­ house. Agrobacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that tra...

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