Introduction The purpose of a seed train is the generation of an adequate number of cells for the inoculation of a production bioreactor. This is timeand cost-intensive: From volumes used for cell thawing or cell line maintenance the cell number has to be increased. The cells are usually run through many cultivation systems which become larger with each passage (e.g. T-flasks, roller bottles or...

Although bioreactor landfills have many advantages associated with them, challenges remain, including the persistence of ammonia-nitrogen in the leachate. It has been suggested that ammonia-nitrogen is one of the most significant long-term pollution problem in landfills and is likely a parameter that will determine when landfill postclosure monitoring may end. The fate of nitrogen in bioreactor...

Background Development of bioprocesses for mammalian cells has to deal with different bioreactor types and scales. Bio-reactors might be intended for seed train and production, research, process development, validation or transfer purposes. During these activities, not only the problem of upand downscaling might lead to failure of repro-ducibility, but also the use of different bioreactor geome...

Formaldehyde (FA) is a very toxic pollutant. FA in indoor air has a negative effect on human health and should be removed by ventilation or by conversion to non-toxic products. The formaldehyde dehydrogenase (FdDH), a NADand glutathione-dependent enzyme from recombinant methylotrophic yeast Hansenula polymorpha, was tested for its ability to oxidize airborne FA. A continuous fluidized bed biore...

For the first time three different natural compounds, isolated from hairy roots of Astragalus membranaceus, cultivated in airlift bioreactor were tested for their cytotoxic potential and apoptosis induction in a panel of human tumor cell lines. Root cultures, cultivated in bioreactor gave 18.5 g l(-1) dry wt roots with the highest astragaloside production in vitro up to now - 1.64% (astragalosi...

Development of new techniques for in vitro culture is necessary to optimize and reduce the production costs. The aim of this work was to introduce the bioreactor BIB (Bioreactor of Immersion by Bubbles) and compare it with conventional culture and bioreactor RITA (Recipient with Automatized Temporary Immersion) for In vitro growth of M. alternifolia plantlets. Plantlets with 1.0 cm height from ...

To maximize the production of carboxylic acids with open cultures of microbial consortia (reactor microbiomes), we performed experiments to understand which factors affect the community dynamics and performance parameters. We operated six thermophilic (55 °C) bioreactors to test how the factors: (i) biomass pretreatment; (ii) bioreactor operating conditions; and (iii) bioreactor history (after ...

OBJECTIVE Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demon...

OBJECTIVE In this study, we describe a novel bioreactor system to deliver controlled stretch protocols to bioengineered heart muscle (BEHM) constructs. Our primary objective was to evaluate the effect of mechanical stretch on the contractile properties of three-dimensional cardiac constructs in vitro. METHODS BEHMs were formed by culturing primary neonatal cardiac myocytes in a fibrin gel usi...