Background: Mushroom Tyrosinase a potent candidate in clinical studies known as polyphenol oxidase, is a metaloenzyme from the oxidase superfamily widely distributed from lower to higher life forms. It plays a crucial role in sclerotization of exoskeleton in insects, also responsible for skin pigmentation in mammalians. Objective: In this study, after reconstitution of MT by some metal io...

Cinnamaldehyde is an important compound of the cinnamon essential oil, and it is responsible for the most of the health benefits of cinnamon. Enriching foods such as milk with cinnamaldehyde can lead to greater utilization of cinnamaldehyde properties. In this study, we investigated the interaction of cinnamaldehyde with bovine alpha-lactalbumin. Analyzing the spectrum of alpha-lactalbumin in t...

We report the synthesis of highly luminescent, water soluble quantum clusters (QCs) of gold, which are stabilized by an iron binding transferrin family protein, lactoferrin (Lf). The synthesized AuQC@Lf clusters were characterized using UV-Visible spectroscopy, X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), photoluminescence (PL), matrix assisted laser desorptio...

in the present study, we investigated the effect of a new anticancer pd(ii) complex, 1,10-phenanthroline-n-butyl dithiocarbamato pd(ii) nitrate, on the heme releasing from human hemoglobin (hb) as well as alterations in the structure and function of hb using different spectroscopic methods of uv-vis, fluorescence and circular dichroism (cd)at two temperatures of 25 and 37 °c. fluorescence data ...

Chirality of a nucleotide-Cu(II) complex molecule, [CuNa(GMP)(HGMP)(H(2)O)(7)]·6(H(2)O)·CH(3)OH (GMP = guanosine 5'-monophosphate), is delivered to the three-dimensional supramolecular architecture by hydrogen bonding. Liquid- and solid-state circular dichroism (CD) spectroscopy are used to analyse the molecular and supramolecular chirality.

Background: DAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is co...

Circular dichroism (CD) spectroscopy is a powerful technique employed to study the structure of biomolecules. More accurate calculation CD from first principles will aid both computational and experimental studies protein dynamics. We apply diabatisation scheme improve description nearest neighbour interactions between two electronic transitions (n?* ?nb?*) localised on each individual peptide ...

A short peptide corresponding to the alpha-helical region of BPTI shows partial folding in aqueous solution (pH 7) as judged by circular dichroism (CD). Folding is temperature and denaturant sensitive, and the peptide is monomeric. The difference CD spectrum, obtained from spectra at two temperatures, indicates that the peptide folds as an alpha-helix. Difference CD spectroscopy provides a sens...

The hexadentate, and ditopic ligand 2,5-bis([2,2']bipyridin-6-yl)pyrazine yields a chiral, tetrameric, square-shaped, self-assembled species upon complexation with Fe2+ ions. The racemate of this complex was resolved with antimonyl tatrate as the chiral auxiliary. The purity of the enantiomer was determined by NMR spectroscopy, by using a chiral, diamagnetic shift reagent, and by circular dichr...

Chirality, the absence of mirror symmetry, governs behavior in most biologically important molecules, thus making chiral recognition great importance pharmaceutical and agrochemical industries, as well medicine. Chiral molecules can be characterized by means optical experiments based on chiro-optical excitation molecules. Specifically, absorptive materials differently absorb left- right-circula...