We have isolated mutations in AraC protein that specifically block either induction or repression at the ara pBAD promoter. These hemiplegic mutations identify amino acid residues that, correspondingly, are involved only in the induction or only in the repression activities of the protein. Residues key only for induction are 13, 15, and 18, which are located in the N-terminal arm of AraC, and r...

A lambda lysogen with the prophage inserted into the arabinose B gene of Escherichia coli strain K-12 has been prepared. Induction of the phage from this lysogen yields viable phage at a frequency 4 X 10(-6) that found for induction of lysogens with phage inserted at the normal attachment site. Over 30% of the phage particles induced from the insertion in ara are arabinose-transducing phage. Th...

BACKGROUND Specific transcription factors (TFs) modulate cardiac gene expression in murine models of heart failure, but their relevance in human subjects remains untested. We developed and applied a computational approach called transcriptional genomics to test the hypothesis that a discrete set of cardiac TFs is associated with human heart failure. METHODS AND RESULTS RNA isolates from faili...

Axenfeld Rieger Syndrome is anterior segment dysgenesis characterized by posterior embryotoxon and of the chamber. Developmental abnormalities angle cause increased resistance to outflow. Ocular hypertension a complication in almost 50% cases. It an autosomal dominant disease its prevalence between 50,000 100,000 newborns per year. The main associated affected genes are FOXC1 PITX2, occurring 4...

BACKGROUND Diminished ovarian reserve (DOR) is one of the causes of infertility in young women. In this prospective study, gene expression profiling (GEP) of corona radiata cells (CRC) was performed to identify genes deregulated in DOR patients. METHODS Microarray-based GEP of CRC isolated from eight women undergoing IVF was performed to identify genes differentially expressed between patient...

ÖZET Glokom, optik sinirin dejenerasyonu ile karakterize karmaşık bir hastalıktır ve dünya çapında geri döndürülemez körlüğün en yaygın nedenidir. Epidemiyolojik çalışmalar, glokomdan etkilenen ailelerin raporları, genom ilişkilendirme çalışmaları glokomun hayvan modelleri kullanılarak glokom için genetik temel oluşturulmuştur. nadir görülen erken başlangıçlı hastalık (40 yaşından önce) tipik o...