A dicistronic minigenome containing the M-F gene junction was used to determine the role of the simian virus 5 (SV5) intergenic regions in transcription. The M-F junction differs from the other SV5 junctions by having a short M gene end U tract of only four residues (U4 tract) and a 22-base M-F intergenic sequence between the M gene end and F gene start site. Replacing the 22-base M-F intergeni...

Helical junctions are ubiquitous structural elements that govern the folding and tertiary structure of RNAs. The tobacco ringspot virus hairpin ribozyme consists of two helix-loop-helix elements that lie on adjacent arms of a four-way junction. In the active form of the hairpin ribozyme, the loops are in proximity. The nature of the helical junction determines the stability of the hairpin riboz...

Background and Aim: This study aimed to assess the effect of G-Bond and Z-Prime Plus on fracture resistance of prefabricated zirconia posts bonded to root canal walls. Materials and Methods: This in-vitro experimental study evaluated 22 mandibular premolars with equal diameter and length. The teeth were cut at the cementoenamel junction (CEJ), underwent root canal treatment, and were randomly ...

The activity of gap junction channels between cultured and clustered carotid body glomus cells of the rat was studied with dual voltage clamping during normoxia (PO(2) 300 Torr) and hypoxia induced by sodium dithionite (Na(2)S(2)O(4)) or 100% N(2). Na(2)S(2)O(4) reduced the saline PO(2) to approximately 10 Torr, whereas 100% N(2) reduced ambient O(2) to approximately 60 Torr. The following obse...

Generation-recombination ~g-r! noise originated by the fluctuating occupancy of deep level traps in the depletion regions of a junction field effect transistor ~JFET! has been thoroughly analyzed. A numerical simulator of this type of device, which allows us to calculate all relevant electrical magnitudes in the structure, was used to calculate the noise power spectral density. To check our sim...

In this article we consider a simple model for a c–axis Pb/YBa2Cu3O7−δ Josephson junction. The observation of a nonzero current in such a junction by Sun et al. [A. G. Sun, D. A. Gajewski, M. B. Maple, R. C. Dynes, Phys. Rev. Lett. 72, 2267 (1994)] has been taken as evidence against d–wave superconductivity in YBa2Cu3O7−δ. We suggest, however, that the pairing interaction in the CuO2 planes may...