نتایج جستجو برای: gene disruption

تعداد نتایج: 1185457  

2014
Shahida Shahana Delma S. Childers Elizabeth R. Ballou Iryna Bohovych Frank C. Odds Neil A. R. Gow Alistair J. P. Brown

Precise genome modification is essential for the molecular dissection of Candida albicans, and is yielding invaluable information about the roles of specific gene functions in this major fungal pathogen of humans. C. albicans is naturally diploid, unable to undergo meiosis, and utilizes a non-canonical genetic code. Hence, specialized tools have had to be developed for gene disruption in C. alb...

Journal: :Journal of bacteriology 2003
Takaaki Sato Toshiaki Fukui Haruyuki Atomi Tadayuki Imanaka

In contrast to the high accumulation in sequence data for hyperthermophilic archaea, methodology for genetically manipulating these strains is still at an early stage. This study aimed to develop a gene disruption system for the hyperthermophilic euryarchaeon Thermococcus kodakaraensis KOD1. Uracil-auxotrophic mutants with mutations in the orotidine-5'-monophosphate decarboxylase gene (pyrF) we...

Journal: :Japanese Journal of Thrombosis and Hemostasis 1999

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