there are many techniques to knock out directed genes in bacteria, some of which have been described in salmonella species. in this study, a combination of soeing pcr method and the λ red disruption system were used to disrupt phop gene in wild type and standard strains of salmonella typhimurium. three standards pcr and one fusion pcr reactions were performed to construct a linear dna including...

background: among all common techniques in sitedirectedmutagenesis, λ red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. in thismethod, there is always the risk of dna linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. objectives:to overcome this, we constructeda recombinant vector to disrupt phop gene in salmonella...

There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEing PCR method and the λ Red disruption system were used to disrupt phoP gene in wild type and standard strains of Salmonella typhimurium. Three standards PCR and one fusion PCR reactions were performed to construct a linear DNA including...

Abstract Gene targeting is useful to isolate strains with mutations in a gene of interest for efficient breeding. In this study, we generated msh4 or mer3 single-gene disruptant monokaryons using Pleurotus ostreatus Δ ku80 strain targeting. Dikaryons P. ×Δ were isolated via backcrosses, and the number basidiospores produced was measured. The fell by an average 1/13.7 dikaryons versus + dikaryon...

uterine leiomyomas/fibroids are the most common pelvic tumors of the female genital tract. the initiators remaining unknown, estrogens and progesterone are considered as promoters of fibroid growth. fibroids are monoclonal tumors showing 40-50% karyo-typically detectable chromosomal abnormalities. cytogenetic aberrations involving chromosomes 6, 7, 12 and 14 constitute the major chromosome abno...

The fatty aldehyde dehydrogenase (FALDH) ALDH3A2 is the causative gene of Sjögren-Larrson syndrome (SLS). To date, the molecular mechanism underlying the symptoms characterizing SLS has been poorly understood. Using Aldh3a2 mice, we here found that Aldh3a2 was the major FALDH active in undifferentiated keratinocytes. Long-chain base metabolism was greatly impaired in Aldh3a2 keratinocytes. Phen...