The promoters and plasmids of Corynebacterium glutamicum can be viewed as subjects for genetic examination, tools for studies of gene expression, and a means for the improvement of industrial strains. Various classes of C. glutamicum promoters, their specific functions in gene expression control, and methods for their analysis are described in this review. C. glutamicum promoters recognized by ...

The vector borne diseases cause heavy loss to mankind and livestock industry throughout the world. Global climate changes reported to contribute to the recurrence and new epidemics of vector borne diseases. Unfortunately, the available strategies to control vector borne diseases are insufficient and public health burden of the major vector borne diseases is on increasing trend. Complete eradica...

background: dengue is amongst the most serious mosquito-borne infectious disease with hot spots in tropical and subtropical parts of the world. unfortunately, no licensed vaccine for the disease is currently available in medicine markets. the only option available is the management of dengue vector mosquito, aedes aegypti (diptera: culicidae). method: predatory potential of five odonate nymphs ...

INTRODUCTION Current approaches to understanding the progression of Pierce’s disease are limited by the lack of genetic techniques that can be used to study the biology of Xylella fastidiosa (Xf). In particular, extrachromosomal elements, such as plasmids, having long-term stability in Xf when grown in lab cultures or en planta, have not yet been satisfactorily developed. We will develop vector...

Description of ESM included in this file: 1) Annotated sequence maps of the pSOS, pSOX, and pSOS-X vectors 2) A spreadsheet listing the genetic elements of the above vectors 3) Several tables indicating the outcome of our various optimization efforts 4) Expanded Materials and Methods covering those optimizations and other routine molecular biology steps

Despite that Vibrio spp. have a significant impact on the health of humans and aquatic animals, the molecular basis of their pathogenesis is little known, mainly due to the limited genetic tools for the functional research of genes in Vibrio. In some cases, deletion of target DNAs in Vibrio can be achieved through the use of suicide vectors. However, these strategies are time-consuming and lack...

Recombinant adeno-associated viruses (rAAV) are highly efficient vectors for gene delivery into the central nervous system (CNS). However, host inflammatory and immune responses may play a critical role in limiting the use of rAAV vectors for gene therapy and functional genomic studies in vivo. Here, we evaluated the effect of repeated injections of five rAAV vectors expressing different geneti...