نتایج جستجو برای: genome dna extraction
تعداد نتایج: 836636 فیلتر نتایج به سال:
The NucliSens Extractor is an automated nucleic acid isolation system based on guanidinium thiocyanate (GuSCN)-silica extraction technology. The system has been validated for the isolation of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) RNAs from human samples in combination with nucleic acid sequence-based amplification- and reverse transcription-PCR-based methods. We evaluat...
background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...
The European Prospective Investigation into Cancer and nutrition (EPIC) is a long-term, multi-centric prospective study in Europe investigating the relationships between cancer and nutrition. This study has served as a basis for a number of Genome-Wide Association Studies (GWAS) and other types of genetic analyses. Over a period of 5 years, 52,256 EPIC DNA samples have been extracted using an a...
dna extraction with an appropriate quality and quantity is the first step in molecular identification of some group of insects that their identification morphologically is difficult and dependent on a particular life stage, size or gender. selection of a suitable protocol is a key factor to provide a pure dna. in this study quality and quantity of five extraction methods used in entomological l...
Local Sudanese sheep populations inhabiting diverse environmental conditions and holding opposing morphologies provide opportunities for molecular-genetic research. Characterizing their genome is crucial sustainable breeding improvement targeting favorable genes in programs. However, the of these populations, which comprises several subtypes, remains uncharacterized using whole-genome sequence ...
background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...
Graphical representation of DNA sequences is one of the most popular techniques for alignment-free sequence comparison. Here, we propose a new method for the feature extraction of DNA sequences represented by binary images, by estimating the similarity between DNA sequences using the frequency histograms of local bitmap patterns of images. Our method shows linear time complexity for the length ...
the dna molecule has been known to be the cellular target for many cytotoxic anticancer agents for several decades. understanding how drug molecules interact with dna has become an active research area in the interface between chemistry, molecular biology and medicine. dna extraction has been suggested as a main step affecting molecular dna technology such as pcr and pcr-based methods. therefor...
Millions of scientific specimens are housed in museum collections, a large part which fluid preserved. The use formaldehyde as fixative and subsequent storage ethanol is especially common ichthyology herpetology. This type preservation damages DNA reduces the chance successful retrieval genetic data. We applied ancient extraction single stranded library construction protocols to variety vertebr...
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