background :  sequence-based identification of various microorganisms including archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template dna is yielded and it can be used in polymerase chain reactions (pcr). considering the fact that dna extraction from these microorganisms is time consu...

Background and Aims: Multiple Sclerosis is a chronic and autoimmune disease, which causes inflammation and demyelination of the axons in CNS. Studies have indicated that Herpesviruses might be one of the potential environmental elements that can trigger the initiation of the inflammatory cascade in MS. The purpose of this study was to determine whether the genome of CMV and (or) EBV is present ...

In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...

in diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. if not inactivated properly, cellular and/or environmental dna nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in pcr experiments. when crude cell extracts, proteinase k–treat...

Hybrids were produced by crossing female Caspian kutum Rutilus frisii kutum with male grass carp Ctenopharyngodon idella. The genome of eight larvae and parents were studied using microsatellite markers for genetic evaluation and verification. After DNA extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers. Hybridizie...

hybrids were produced by crossing female caspian kutum rutilus frisii kutum with male grass carp ctenopharyngodon idella. the genome of eight larvae and parents were studied using microsatellite markers for genetic evaluation and verification. after dna extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers. hybridizie...

Typical DNA extraction protocols from commercially available kits provide an adequate amount of DNA from a single individual mosquito sufficient for PCR-based assays. However, next-generation sequencing applications and high-throughput SNP genotyping assays exposed the limitation of DNA quantity one usually gets from a single individual mosquito. Whole genome amplification could alleviate the i...

medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. these compounds especially antioxidants can increase oxidation of dna in response to wounds. nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationsh...