A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

Tuberculosis (TB) is one of the oldest disease known to humanity and among deadliest epidemics in top 10 causes death worldwide. The incidence tuberculosis India has dramatically increased contributing it Human Immunodeficiency Virus (HIV). Various diagnostic methods are available for detection tubercular bacilli. Only full proof criteria diagnosis TB demonstrate bacilli from affected tissue. T...

BACKGROUND AND AIMS Abnormal glucagon secretion and functional alterations of the exocrine pancreas have been described in patients with type 1 diabetes (T1D), but their respective anatomical substrata have seldom been investigated. Our aim was to develop an automated morphometric analysis process to characterize the anatomy of α-cell and exocrine pancreas in patients with T1D, using the public...

In the coutrse of a prospective, controlled evaluation of the effect of portacaval anastomosis on hepatic lhemosidlerin deposition(l), a large number of iron-stained slicles were evaltuated blindly, semniquantitatively and serially. Those slides in wlhichi excessive deposition of hemosiderin was present microscopically appeared blue in color to gross inspection while those in whiom little or no...

Introduction:Fine Needle Aspiration Cytology (FNAC) is used widely for the preliminary pre-operative diagnosis of all superficial palpable lesions and for deep seated lesions under ultrasound guidance. Though Pap stain, May GrunwaldGiemsa (MGG) and Haematoxylin and Eosin (H & E) stains are used commonly, Leishman stain provides good cytoplasmic and background details. Hence, it can beused in ad...

BACKGROUND/AIM Cancer cells have an essential demand for vitamin B12 (cobalamin) to enable cellular replication. The present pilot study quantified the immunohistochemical expression of vitamin B12 transport protein (Transcobalamin II; TCII), cell surface receptor (Transcobalamin II-R; TCII-R) and proliferation protein (Ki-67) in human tumor xenografts. MATERIALS AND METHODS Tissue microarray...

Interpretation of the protected specimen brush (PSB) technique is based on quantitative bacterial cultures (QC), which unfortunately requires at least 24 h. We prospectively compared the diagnostic value of direct examination (DE) and QC of PSB specimens in 75 patients with suspected pneumonia. We also determined the optimal technique for DE. QC was performed using the serial dilution technique...