The first dimeric catalase-peroxidase of eucaryotic origin, an intracellular hydroperoxidase from Penicillium simplicissimum which exhibited both catalase and peroxidase activities, has been isolated. The enzyme has an apparent molecular mass of about 170 kDa and is composed of two identical subunits. The purified protein has a pH optimum for catalase activity at 6.4 and for peroxidase at 5.4. ...

Plant peroxidases are involved in numerous cellular processes in plant development and stress responses. Four plasma membrane-bound peroxidases have been identified and characterized in maize (Zea mays L.) roots. In the present study, maize seedlings were treated with different stresses and signal compounds, and a functional analysis of these membrane-bound class III peroxidases (pmPOX1, pmPOX2...

Abstract The purpose of the study was to pretreat fermented rooibos biomass with partially purified horseradish peroxidase (HRP) for lignin removal and convert delignified soluble sugars through saccharification a formulated holocellulolytic enzyme cocktail (HEC). HRP extracted from root tissue by membrane filters characterised biochemically. used remove before hydrolysing it HEC. Our findings ...

The plausible role of arginine and tyrosine residues at the active side of horseradish peroxidase (HRP) in aromatic donor (guaiacol) oxidation was probed by chemical modification followed by characterization of the modified enzyme. The arginine-specific reagents phenylglyoxal (PGO), 2,3-butanedione and 1,2-cyclohexanedione all inactivated the enzyme, following pseudo-first-order kinetics with s...

Background: Withania somnifera L. Dunal, (Ashwagandha) belongs to the family Solanaceae and is known as ‘Indian ginseng’ in the traditional system of medicine. Objectives: In the present investigation, comparative analysis was carried out between two different ecotypes of W. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (CAT), s...

The effects of exogenous abscisic acid (ABA) and its two agonists, Sulfacetamide (Sa) and Sulfasalazine (SS) on tolerance of tomato (Lycopersicon esculentum Mill. Cv. Super chief) under drought stress were studied. Eight-week plants were treated with ABA (25 and 50 mg/L), Sulfacetamide (Sa) (25, 50 and 100 mg/L) and Sulfasalazine (SS) (25, 50 and 100 mg/L). Solutions were sprayed daily and samp...

A brief study to analyze oxidative stress and antioxidant response of cadmium stress was carried out. The plant chosen Vigna radiata (var. SML-668) was hydroponically grown in 0, 50μM, 100μM and 150μM, cadmium chloride. The roots were taken from hydroponic culture at 6 days of growth for various enzymatic and metabolic estimations. Among the antioxidant enzymes, root catalase and ascorbate pero...