Methanol is a versatile compound that can be biologically synthesized from methane (CH4) by methanotrophs using a low energy-consuming and environment-friendly process. Methylocella tundrae is a type II methanotroph that can utilize CH4 as a carbon and energy source. Methanol is produced in the first step of the metabolic pathway of methanotrophs and is further oxidized into formaldehyde. Sever...

urease was immobilized on platinum electrode both by chemical binding and electropolymerization.the conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 m and linear dynamic range from 4.9×10-5 to 5.8×10-3 m for urea concentration when the enzyme is covalently immobilized on pt electrodes. conductometric transducers respond to the changes in ionic strength thereby le...

Single cell whole genome amplification is susceptible to amplification biases that impact the accuracy of single cell sequencing data. To address this, we have developed a microfluidic device for the isolation and purification of genomic DNA from individual cells. The device uses a micropillar array to physically capture single cells and its chromosomal DNA upon extraction. The extracted DNA is...

Enzyme cost is the major problem for industrial scale application. Immobilization is a promising approach to moderate the enzyme cost factor and increase its stability and activity. In this study, sol-gel method was used to prepare the immobilization platform and entrapped lipase as one of the most used enzyme in dairy processing, cosmetics and pharmaceutical industries. Lipase from Candida rug...

This paper describes the incorporation of DNA aptamers with a microfluidic device for the isolation of cancer cells from whole blood. Aptamers with specific binding to leukemia or colorectal cells were used as an alternative to antibodies that are often used for circulating tumor cell (CTC) sorting. The microfluidic device contained unique geometry and design of micropillars, and showed better ...

HIV has caused a global pandemic over the last three decades. There is an unmet need to develop point-of-care (POC) viral load diagnostics to initiate and monitor antiretroviral treatment in resource-constrained settings. Particularly, geographical distribution of HIV subtypes poses significant challenges for POC immunoassays. Here, we demonstrated a microfluidic device that can effectively cap...

Intracellular recording, which allows direct measurement of the membrane potential and currents of individual neurons, requires a very mechanically stable preparation and has thus been limited to in vitro and head-immobilized in vivo experiments. This restriction constitutes a major obstacle for linking cellular and synaptic physiology with animal behavior. To overcome this limitation we have d...

We expressed and purified a recombinant P. pyralis luciferase with N-terminal His-tags. The silanized Ni or Cu-loaded magnetic particles were prepared and used to assemble the His-tagged P. pyralis luciferase. This enzyme immobilized on functionalized magnetic nanoparticles (MNPs) via electrostatic interactions of His-tag with Ni2+/Cu2+ ions on the surface of MNPs using si...

OBJECTIVE Ibrutinib is an irreversible Bruton tyrosine kinase inhibitor approved for treatment of Waldenstrom macroglobulinemia, chronic lymphocytic leukemia, and mantle cell lymphoma that increases the risk of bleeding among patients. Platelets from ibrutinib-treated patients exhibit deficiencies in collagen-evoked signaling in suspension; however, the significance of this observation and how ...

Three-dimensional gel-based microchips with immobilized proteins were used for quantitative immunoassay of a series of plant (ricin and viscumin) and bacterial (staphylococcal enterotoxin B, tetanus and diphtheria toxins, and lethal factor of anthrax) toxins. It was shown that different types of immunoassays (direct, competitive, and sandwich type) could be carried out on gel microchips. As sho...