Background: Cholera toxin B subunit (CTB) has been extensively considered as an immunogenic and adjuvant protein, but its yield of expression is not satisfactory in many studies. The aim of this study was to compare the expression of native and mutant recombinant CTB (rCTB) in pQE vector. Methods: ctxB fragment from Vibrio cholerae O1 ATCC14035 containing the substitution of mutant ctxB for ami...

Precedence constrained sequencing problem (PCSP) is related to locate the optimal sequence with the shortest traveling time among all feasible sequences. In PCSP, precedence relations determine sequence of traveling between any two nodes. Various methods and algorithms for effectively solving the PCSP have been suggested. In this paper we propose a cuckoo search algorithm (CSA) for effectively ...

the aim of this paper is to introduce and study a new concept ofstrong double $(a)_ {delta}$-convergent sequence offuzzy numbers with respect to an orlicz function and also someproperties of the resulting sequence spaces of fuzzy   numbers areexamined. in addition, we define the double$(a,delta)$-statistical convergence of fuzzy  numbers andestablish some connections between the spaces of stron...

background: lipophosphoglycan 3 (lpg3) is required for the lpg assembly, a well known virulent molecule. in this study, the lpg3 gene of the lizard and mammalian leishmania species were cloned and sequenced. a three-dimensional structure (3d) for the target sequence was also predicted by comparative (homology) modeling. materials and methods: an optimization pcr amplification was performed on g...

systemic lupus erythematosus (sle) is an autoimmune disease in which polymorphisms within the human leukocyte antigen (hla) region have been associated to its etiology. we conducted this study to compare the hla-dqb1 allelic sequence variation among sle patients and controls in the northeast of iran. genomic dna of 40 sle patients and 83 healthy controls were amplified by polymerase chain react...

pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. a polymerase chain reaction (pcr) assay was developed using primers derived from conserved part of 16s-23s rrna gene. the pcr amplified a fragment size of 0.7 kb using dna from nine avian p. multocida  isolates...

A sequence of Permian rocks crops out in Northwest of Khur in Central Alborz, North of Iran. This sequence consists of the Dorud (Asselian-Sakmarian), Ruteh (Artinskian-Murgabian) and Nesen (Early Djulfian) formations. The Ruteh and Nesen formations in Alborz Range were deposited in a shallow marine environment of the continental margin in the Paleo-Tethys. The Ruteh Formation with a thickness ...