Studies have been carried out to determine the maximal solubilization of cholesteryl oleate and tocopheryl acetate within mixed bile salt-fatty acid micelles and to establish reproducible assays for pancreatic esterase activity using these micellar substrates. At pH 8.5, using 30 mM sodium taurocholate and 10 mM oleic acid, reproducible micellar solutions of the esters could be prepared giving ...

In order to achieve prolong corneal contact time of norfloxacin(NFX) for treatment of extra ocular diseases, a pH triggered nanoparticulate in situ gelling system was designed to explore dual advantage of nanoparticles and in situ gelling system, for its ocular delivery. NFX loaded nanocarriers were developed by ionotropic gelation technique using chitosan as a matrix forming polymer, cross-lin...

In order to achieve prolong corneal contact time of norfloxacin(NFX) for treatment of extra ocular diseases, a pH triggered nanoparticulate in situ gelling system was designed to explore dual advantage of nanoparticles and in situ gelling system, for its ocular delivery. NFX loaded nanocarriers were developed by ionotropic gelation technique using chitosan as a matrix forming polymer, cross-lin...

purpose : to investigate the sensations experienced by chinese patients during refractive surgery including the laser in situ keratomileusis (lasik), epi-lasik, and sub-bowman keratomileusis (sbk) techniques. a prospective, randomized, and self-matched clinical study was conducted. methods : the participants included 44 men and 58 women aged 16 to 47 years old (mean 24.3±0.6 years) who underwen...

Site-directed mutagenesis is used to identify amino acid residues that dictate reported differences in substrate specificity between rat hepatic neutral cytosolic cholesteryl ester hydrolase (hncCEH) and rat lung carboxylesterase (LCE), proteins differing by only 4 residues in their primary sequences. Beginning with LCE, the substitution Met(423) --> Ile(423) alone or in combination with other ...

p-Nitrophenyl and cholesteryl-N-alkyl carbamates are good inhibitors of porcine pancreatic cholesterol esterase-catalyzed hydrolysis of p-nitrophenyl butyrate. p-Nitrophenyl-N-butyl and N-octyl carbamates (compounds 1 and 2, respectively) are potent active site-directed irreversible inhibitors of this enzyme. The inhibition of cholesterol esterase by compound 1 or 2 shows saturation kinetics wi...

Abstract. Witasari LD, Huang FC, Schwab W. 2022. Identification and characterization of strawberry FvGELP1 (Fragaria vesca GDSL esterase/lipase).Biodiversitas 23: 907-915. Fruit softening is primarily the result hydrolytic enzymes activity. Esterases are reasonable candidates due to their putative role in cell wall components degradation. This study aimed identify characterize a new enzyme rela...