Background: The use of C-type natriuretic peptide (CNP) combined with cysteamine during pre-in vitro maturation (IVM) can help establish an effective pre-IVM system. Aims: This study was designed to investigate the effects of CNP combined with cysteamine on meiotic maturation and developmental competence of bovine oocytes in vitro. Met...

Background: Cryopreservation of oocytes is an effective technology in assisted reproductive technology. Although successful vitrification of gametes has been reported in several mammalian species, the survival is generally low. The aim of this study was to investigate the effects of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) on preantral follicle development after vitrifi...

Complete functional oocyte maturation involves many complex processes that occur within preovulatory follicles and are initiated and sustained by the gonadotropins, steroid hormones and many recognized and unrecognized factors. The product of oocyte maturation is a mature egg that has complete nuclear, plasma membrane and cytoplasmic changes that enable normal fertilization and subsequent devel...

the aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (cocs). bovine ovaries were collected from the local slaughterhousein a container with 30-37°c physiologic saline and supplemented with antibiotics. the grade a aspiratedcocs were assigned to 6 experimental groups; the 1st group was control (n=100) in whi...

Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop and their quality is inferior to that of in vivo derived blastocysts. It is unknown whether the cytoplasmic maturation of the oocyte is sufficiently sustained in current in vitro maturatio...

Wen-Shiun Yueh and Ching-Fong Chang (2000) Morphological changes and competence of maturing oocytes in the protandrous black porgy, Acanthopagrus schlegeli. Zoological Studies 39(2): 114-122. The study objectives aimed to investigate the morphological changes of oocytes during final oocyte maturation and ovulation, and the maturational competence of oocytes to respond to maturation-inducing ste...

The aim of the present study was to find the effects of pre and post vitrification taxol treatment on bovineimmature cumulus oocyte complexes (COCs). Bovine ovaries were collected from the local slaughterhousein a container with 30-37°C physiologic saline and supplemented with antibiotics. The grade A aspiratedCOCs were assigned to 6 experimental groups; The 1st group was control (n=100) in whi...

The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...

Background: Different culture media are used for in vitro maturation of ovine oocyte. The Weymouth medium are used for in vitro maturation of rat and mice oocytes with very low levels of supplementation (Just FBS, while to acquire the good results in TCM hormone, and serum supplementation and in some cases growth factor are necessary to gain the optimized results. The aim of the present study w...