BACKGROUND Human amnion membrane (HAM) was suggested to be a superior antigenic substrate for immunoblotting in detecting autoantibodies of autoimmune bullous skin diseases. OBJECTIVES To determine the properties of HAM as an antigenic substrate for the detection of autoantibodies in pemphigus vulgaris and bullous pemphigoid. METHODS Immunomapping and tandem liquid chromatography mass spect...

background: cross-contamination between cells is a usual mistake at cell culture laboratories and cell banks worldwide. mrc5 diploid cell and rk-13, vero and hela continuous cell lines are used in different stages of human viral vaccines propagation at razi vaccine and serum research institute of iran. however diploid and continuous cells are propagated at separated cell culture laboratory and ...

Results of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method...

INTRODUCTION Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases. For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) ba...

Using the indirect hemagglutination (IH), indirect immunofluorescence (IIF) and enzyme linked immunosorbent assay (ELISA) tests for the diagnosis of Chagas disease, 4000 serum samples were examined. This study was conducted with different purposes: clinical interest, research support and parasitological monitoring of those patients with Chagas disease who were treated with heart transplantation...

BACKGROUND Immunofluorescence testing is an important tool for diagnosing blistering diseases. OBJECTIVE To characterize the immunofluorescence findings in patients diagnosed with autoimmune blistering skin diseases. METHODS We retrospectively analyzed immunofluorescence results encompassing a 10-year period. RESULTS 421 patients were included and divided into 2 groups: group 1- intraepid...