I N 1963, FINK AND MALMGREN,1 using the technic of immunofluorescence, showed that lymphoid tissue in muring leukemia contains antigen reactive with specific anti-viral antibody. Further studies by Fink, Malmgren, Rauscher, Orr and Karon2 demonstrated that some human leukemic blood and bone marrow cells also reacted with the murine leukemic virus ( Rauscher) antibody. In addition, rabbit antise...

Background: The genus Calophyllum and its species has received great research interest for their phytochemical content therapeutic potential. Said was sparked by the discovery of compounds with anti-HIV activities in one extracts since been studied potential treating other morbidities. Generally, under contain various coumarins, xanthones, triterpenoids, steroids, chromanones. Extracts said bio...

Mice with severe combined immunodeficiency (SCID) provide a model system to examine the in vivo homing, engraftment, and growth patterns of normal and malignant human hematopoietic cells. The relation between leukemic cell growth in this model and the treatment outcome in patients from whom cells were derived has not been established. Leukemic cells from 42 children with newly diagnosed high-ri...

Acute myeloid leukemias (AMLs) are monoclonal proliferations of undifferentiated myeloid progenitors in blood and bone marrow. Long-term remissions are achieved in <50% of patients. There is hope that activation of specific antileukemic immune responses could efficiently eliminate minimal residual disease at the end of chemotherapy and decrease the frequency of relapses. It was demonstrated tha...

Soft-agar cloning was used to investigate possible granulopoietic-monopoietic regulatory defects in the chick with myeloblastic leukemia induced by avian myeloblastosis virus. The plasma levels of granulocyte-monocyte colony-stimulating activity (CSA) of normal and leukemic plasmas were the same when undiluted or unfractionated plasmas were tested. However, dilution or fractionation revealed el...

The number of bone marrow fibroblast colony formations decreases in most cases of acute leukemia before the initiation of chemotherapy. This study was undertaken to clarify the mechanism of suppression of fibroblast colony formation in leukemic patients. Titration of the number of bone marrow cells did not indicate a linear relationship between the number of bone marrow cells cultured and the n...

The Na(+)/H(+) exchanger isoform 1 (NHE1) is primarily responsible for the regulation of intracellular pH (pH(i)). It is a ubiquitous, amiloride-sensitive, growth factor-activatable exchanger whose role has been implicated in cell-cycle regulation, apoptosis, and neoplasia. Here we demonstrate that leukemic cell lines and peripheral blood from primary patient leukemic samples exhibit a constitu...

The receptor-mediated degradation of 125I-low density lipoprotein (LDL) was compared in normal white blood cells and leukemic cells. The cells were isolated from the peripheral blood and bone marrow of healthy subjects and patients with newly diagnosed leukemia. The cells from most of the 40 consecutive patients with acute myelogenous leukemia showed markedly higher degradation rates as compare...

Leukemic blasts from patients with acute nonlymphoid leukemia were examined for the presence of Ig, receptors for IgGFc, and for their capacity to mediate antibody-dependent cellular cytotoxicity (ADCC) against chicken red blood cells (RBC) coated with IgG and spontaneous cell-mediated cytotoxicity (SCMC) against cells of K562 cell line. Leukemic blasts from acute myeloblastic leukemia (AML) pa...

Growth factor-independent clones of myeloid leukemic cells can regain a growth factor-dependent state during differentiation. Loss of viability in these differentiating leukemic cells in the absence of growth factor was associated with DNA fragmentation and morphologic changes typical of programmed cell death (apoptosis). The differentiating leukemic cells could be rescued from apoptosis by a h...