A new method is described to enrich genomic libraries for clones containing microsatellite repeats. The method involves selection on completed M13 genomic libraries rather than on genomic DNA before library construction. It uses two reactions, in which microsatellite oligonucleotides prime strand extension. The first reaction uses a biotinylated primer allowing vectors with microsatellite-conta...

To determine if threatened brown bear (Ursus arctos) populations of Montana and Wyoming have lower levels of genetic variation than other North American populations, we examined mitochondrial DNA (mtDNA) and nuclear microsatellite DNA diversity in 220 brown bears from 5 areas: Kodiak Island, Alaska; Kluane National Park, Canada; Eastern Slope of the Rockies (East Slope), Canada; Yellowstone eco...

The genomic distribution of the abundant eukaryotic d(GA x TC)(n) DNA microsatellite suggests that it could contribute to DNA recombination. Here, it is shown that this type of microsatellite DNA sequence enhances DNA recombination in SV40 minichromosomes, the rate of homologous DNA recombination increasing by as much as two orders of magnitude in the presence of a d(GA x TC)(22) sequence. This...

BACKGROUND In patients with colorectal carcinoma, insertions or deletions of short sequences of DNA, a phenomenon called microsatellite instability, are observed. AIM To look for microsatellite instability and mutations of MLH1 and MSH2 gene mutations in patients with colorectal carcinoma. MATERIAL AND METHODS Ten patients with sporadic colorectal carcinoma and 31 patients fulfilling criter...

Parentage testing is an important tool in farm animals for genetically determining the accuracy of pedigree information. The objective of the current study was to implication of multiplexing 14 microsatellite markers for routine parentage testings. The twenty-four lambs were crossbred of Ghazel × Baluchi, Ghazel × Baluchi × Merinos, and Baluchi × Moghani × Merinos breeds. The genomic DNA was ex...

  Background and Objectives: Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant cancer predisposition syndrome caused by germ-line mutations in DNA mismatch repair genes. Tumors arising as a result of these mutations display instability in a sequence area known as microsatellites. Studies have shown that some Bethesda markers (BAT25, BAT26) are more efficient than other...

hybrids were produced by crossing female caspian kutum rutilus frisii kutum with male grass carp ctenopharyngodon idella. the genome of eight larvae and parents were studied using microsatellite markers for genetic evaluation and verification. after dna extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two pairs of microsatellite primers. hybridizie...

Somatic instability of variable number of tandem repeat (VNTR or microsatellite) sequences has been described recently in colorectal cancer as an indicator of a novel mechanism of carcinogenesis.' The recent report in Blood by Wada et aI2 suggested that microsatellite instability is a frequent feature in the evolution of chronic myeloid leukemia (CML) from chronic phase to blast crisis, and the...

Thirteen sperm whales were sampled, using sloughed skin, in the Mediterranean Sea during six distinct encounters. Individuals discriminated results of molecular sexing, mitochondrial control region sequencing and microsatellite genotyping (3 loci). Samples from 57 specimens available whale strandings on northern European coasts. The first ~ 200bp DNA (mtDNA) each sample sequenced three differen...