Bioprocesses, which involve biocatalysts for the production of useful compounds, are expected to become a leading player in green chemistry. The first step in bioprocess development is screening for useful biological reactions in the immense number of microorganisms with infinite diversity and versatility. This review introduces some examples of bioprocess development that started from process ...

For a number of years a new scientiJc unity has been developing frotn a synthesis itz which chemistry and physics have become closely lirlked with biology. On the biological side cytologists are concerned with what happens to the nucleo-proteins and the nucleic acids present in plant and animal cells. These compounds are of great interest to biochemists atld biophysicists, and during I953 impor...

A ligand-observed 1H NMR relaxation experiment is introduced for measuring the binding kinetics of low-molecular-weight compounds to their biomolecular targets. We show that this approach, which does not require any isotope labeling, is applicable to ligand-target systems involving proteins and nucleic acids of variable molecular size. The experiment is particularly useful for the systematic in...

The histochemical effects of the lethal concentration that kills 50% of larvae (LC50) of three biological agents, abamectin, Bacillus thuringiensis and spinosad on the carbohydrates (polysaccharides), proteins, nucleic acids and lipids content of the midgut and fat bodies of Culex pipiens 2nd instar larvae were studied. The results showed that the three tested compounds reduced the carbohydrate...

Peptide nucleic acids (PNA) mimic DNA and RNA by forming complementary duplex structures following Watson-Crick base pairing. A set of reporter compounds that bind to DNA by intercalation are known, but these compounds do not intercalate in PNA/DNA hybrid duplexes. Analysis of the hybrid PNA duplexes requires development of reporter compounds that probe their chemical and physical properties. W...

A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by s...

Aims & Scope: Lead compounds show activities by inhibiting or stimulating the functions of biologically relevant molecules (proteins, nucleic acids, carbohydrates and lipids). Researchers are aiming to improve certain features of the indentified lead compounds. Those to become drug candidates should bind specifically to the targets whereas not affect any other important "off-target" molecules t...

Fluorescent base analogues comprise a group of increasingly important molecules for the investigation of nucleic acid structure, dynamics, and interactions with other molecules. Herein, we report on the quantum chemical calculation aided design, synthesis, and characterization of four new putative quadracyclic adenine analogues. The compounds were efficiently synthesized from a common intermedi...

ABSTRACT The occurrence of cross-contamination, which leads to false-positive results when analyzing samples, is an important problem in the PCR laboratory. There are many ways solve this problem, but none them universal. Treatment with aerosol preferable for decontamination large areas complex surfaces. goal study was determine efficiency different modes treatment disinfection and nucleic acid...

cytotoxicity evaluation of daphne mucronata (thymelaeaceae) extract and one of its active purified components, using seven different cancerous cell lines, revealed the strong antiproliferative activity of the plant. using flow cytometry technique, it was found that treatment of the most responsive cells (k562) with the plant extract or the active component inhibited the progression of cells thr...