Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC). Only two strains, both of cattle origin, were positive for the fliC (H7) gene and could be classified as O157 : H7. Nine isolates had stx shiga toxin genes, either stx1 (1 strain), stx2 (4 isolat...

Endophytic actinomycetes were isolated from surface sterilized leaves of Catharanthes roseus (L.) G. Don offamily Apocynaceae. A total of 38 endophytic actinomycetes were recovered on Starch Casein Agar.Among the 38 isolates 20 morphologically different isolates were screened for antibacterial activity againstBacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Pr...

coagulase-negative staphylococci (cns) are a main cause of nosocomial infection. the main purpose of this study was to determination of frequency of cns isolates in in hospitalized patients and their susceptibility pattern to antimicrobial agents. during 11 month study, 65 cns clinical isolates were recovered from hospitalized patients in different wards of hospital. in vitro susceptibility of ...

The bacterium Clavibacter michiganense subsp. nebraskense (Corynebacterium michiganense subsp. nebraskense) was grown in broth cultures and inoculated into corn plants. The plating efficiency of cells from broth cultures was essentially the same on nutrient broth-yeast extract and the semiselective medium for this bacterium, CNS. However, when cells were isolated from Goss bacterial wilt- and b...

OBJECTIVES The aim of this study was to evaluate the occurrence and dissemination of acquired carbapenem-hydrolysing class D beta-lactamase (class D carbapenemase)- and metallo-beta-lactamase (MBL)-encoding genes among Acinetobacter spp. isolates recovered from medical centres in the Asia-Pacific (APAC) region. METHODS During 2006-07, 41 medical centres located in 10 countries in the APAC reg...

Tobacco cyst nematode (Globodera tabacum solanacearum) isolates were collected from 11 locations in Virginia, 3 in North Carolina, and 1 in Maryland. Isolates from each location were maintained and increased on flue-cured tobacco, Nicotiana tabacum cv K326. Plants of flue-cured tobacco cultivars K326 (susceptible) and NC567 (resistant) were each inoculated with 6,000 G. t. solanacearum eggs/pla...

Repetitive DNA motifs with potential variable-number tandem repeats (VNTR) were identified in the genome of Neisseria meningitidis and used to develop a typing method. A total of 146 meningococcal isolates recovered from carriers and patients were studied. These included 82 of the 107 N. meningitidis isolates previously used in the development of multilocus sequence typing (MLST), 45 isolates r...

In a previous study, the seasonal prevalence was reported for stx+ Escherichia coli O157:H7 in feces and on hides and carcasses of cattle at processing. Overall, 1,697 O157:H7 isolates have now been characterized for the incidence of (i) eae(O157), hlyA, stx1, and stx2 in the recovered isolates and (ii) presumptive rough and presumptive nonmotile isolates. Seven O157:H7 isolates (0.4%) lacked s...